Τίτλος:
Development and validation of a simple reversed-phase high-performance liquid chromatography method for the determination of testosterone in serum of males
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
An isocratic high-performance liquid chromatographic method with detection at 234 nm was developed, optimized and validated for the determination of testosterone in human serum. Propylparaben was used as internal standard. A Hypersil BDS RP-C18 column (150 mm × 4.6 mm, 5 μm), was equilibrated with a mobile phase composed of acetonitrile and water (35:65, v/v) and having a flow rate of 1 ml/min. The elution time for testosterone and internal standard was approximately 11.6 and 9.9 min, respectively. Calibration curves of testosterone in serum were linear in the concentration range of 1-20 ng/ml. Limits of detection and quantification in serum were 0.4 and 1.1 ng/ml, respectively. Recovery was greater than 92%. Intra- and inter-day relative standard deviation for testosterone in serum was less than 2.1 and 3.9%, respectively. This method was applied to the determination of testosterone serum levels of 12 healthy males and data were correlated with data obtained using a radioimmunoassay method. © 2005 Elsevier B.V. All rights reserved.
Συγγραφείς:
Loi, V.
Vertzoni, M.
Vryonidou, A.
Phenekos, C.
Περιοδικό:
Journal of Pharmaceutical and Biomedical Analysis
Λέξεις-κλειδιά:
acetonitrile; propyl paraben; testosterone; water, adult; animal experiment; article; blood analysis; calibration; concentration (parameters); concentration response; controlled study; correlation analysis; data analysis; elution; female; flow rate; human; liquid liquid extraction; male; nonhuman; normal human; priority journal; quantitative analysis; radioimmunoassay; reversed phase high performance liquid chromatography; testosterone blood level; validation process; validation study, Acetonitriles; Chromatography, High Pressure Liquid; Humans; Male; Reproducibility of Results; Spectrophotometry, Ultraviolet; Testosterone; Time Factors; Water
DOI:
10.1016/j.jpba.2005.11.021
