Τίτλος:
Effect of different ventilatory strategies on local and systemic cytokine production in intact swine lungs in vivo
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The purpose of the study was to investigate the effect of different ventilatory strategies on local and systemic cytokine production in swine with intact lungs in vivo after 4 h of mechanical ventilation. Twenty-five swine were anesthetized and then randomized into five groups (n = 5): (1) low tidal volume zero PEEP (LVZP); (2) medium tidal volume zero PEEP (MVZP); (3) high tidal volume zero PEEP (HVZP); (4) low tidal volume PEEP (LVP); (4) high tidal volume PEEP (HVP). Respiratory rate was adjusted to maintain normocapnia and fraction of inspired oxygen (FiO2) was 1.0. TNF-α and IL-10 were measured in BALF and serum at baseline, 2 h, and 4 h of MV. One animal in LVZP (2 h) and two in HVP (3 h) group died before the end of the experiment. TNF-α level in BALF was significantly higher in LVZP and LVP at 4 h compared to baseline and the other groups. IL-10 level in BALF was significantly higher in LVP at 4 h compared to baseline and the other groups. There was a statistically significant increase in serum TNF-α levels at 4 h in LVP group compared to baseline and the other groups at 4 h. There was statistically significant increase in serum IL-10 levels in HVZP and LVP groups at 2 and 4 h which was significantly higher compared to the other groups at 4 h. Our results show that a) low volume MV may induce local and systemic pro- and anti-inflammatory cytokine increase b) in the presence of pro-inflammatory cytokine response there is also an anti-inflammatory response in the same compartment (lungs, circulation). c) There maybe loss of alveolar-to-systemic cytokine compartmentalization. © 2006 Elsevier Inc. All rights reserved.
Συγγραφείς:
Myrianthefs, P.
Boutzouka, E.
Venetsanou, K.
Papalois, A.
Kouloukousa, M.
Kittas, C.
Baltopoulos, G.
Περιοδικό:
Vascular Pharmacology
Λέξεις-κλειδιά:
cytokine; interleukin 10; tumor necrosis factor alpha, animal cell; animal experiment; animal tissue; article; artificial ventilation; breathing rate; controlled study; cytokine production; in vivo study; lung; lung ventilation; male; nonhuman; oxygen consumption; positive end expiratory pressure; priority journal; protein blood level; statistical analysis; statistical significance; swine; tidal volume, Animals; Blood Gas Analysis; Bronchoalveolar Lavage Fluid; Cytokines; Interleukin-10; Lung; Male; Pneumonia; Respiration, Artificial; Swine; Time Factors; Tumor Necrosis Factor-alpha
DOI:
10.1016/j.vph.2005.12.004
