Τίτλος:
Proteomic analysis of childhood de novo acute myeloid leukemia and myelodysplastic syndrome/AML: Correlation to molecular and cytogenetic analyses
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The aim of this study was to investigate the progression of myelodysplastic syndrome (MDS) to acute myeloid leukemia (AML) and to provide additional data regarding the proteomic analysis of AML. The protein profiles obtained were correlated to cytogenetic and molecular analyses. Bone marrow (BM) and peripheral blood (PB) samples were obtained during MDS diagnosis, at MDS transformation to AML, at de novo AML diagnosis and 3 months following treatment. As controls, non-leukemic pediatric patients were studied. Cytogenetic and molecular analyses were carried out by G banding and polymerase chain reaction followed by sequencing, respectively. Differential proteomic analysis was performed by two-dimensional gel electrophoresis and protein identification by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. No significant correlations were noted between protein patterns and cytogenetic or molecular analyses. Certain suppressor genes, metabolic enzymes, immunoglobulins and actin-binding proteins were differentially expressed by BM or PB plasma and cell lysates compared to controls. The obtained data showed that vitamin D and gelsolin played contradicting roles in contributing and restraining leukemogenesis, while MOES, EZRI and AIFM1 could be considered as biomarkers for AML. © 2010 Springer-Verlag.
Συγγραφείς:
Braoudaki, M.
Tzortzatou-Stathopoulou, F.
Anagnostopoulos, A.K.
Papathanassiou, C.
Vougas, K.
Karamolegou, K.
Tsangaris, G.T.
Περιοδικό:
Journal of Amino Acids
Λέξεις-κλειδιά:
actin binding protein; acute phase protein; alpha enolase; antithrombin III; apoptosis inducing factor; BCR ABL protein; CD135 antigen; ceruloplasmin; clusterin; complement component C3; gelsolin; guanine nucleotide binding protein; H Ras protein; heat shock protein 70; immunoglobulin; immunoglobulin mu chain; K ras protein; leucine rich alpha2 glycoprotein precursor; mixed lineage leukemia protein; nucleophosmin; peroxiredoxin 2; protein disulfide isomerase; protein n ras; serum amyloid A; serum amyloid P; transcription factor ETV6; transcription factor RUNX1; unclassified drug; vitamin D; vitamin D binding protein, acute granulocytic leukemia; adolescent; article; blood sampling; bone marrow biopsy; cell lysate; child; childhood leukemia; chromosome 12p; chromosome 4q; chromosome 8q; chromosome 9p; chromosome analysis; chromosome G band; chromosome translocation 12; chromosome translocation 4; chromosome translocation 8; chromosome translocation 9; clinical article; disease course; FLT3 gene; fluorescence in situ hybridization; gene expression; genetic analysis; human; human cell; leukemogenesis; matrix assisted laser desorption ionization time of flight mass spectrometry; myelodysplastic syndrome; NPM1 gene; oncogene H ras; oncogene K ras; oncogene N ras; polymerase chain reaction; preschool child; priority journal; protein analysis; proteomics; school child; sequence analysis; tumor suppressor gene; two dimensional gel electrophoresis, Adolescent; Blood; Blood Chemical Analysis; Bone Marrow; Child; Child, Preschool; Chromosome Aberrations; Cytogenetics; Electrophoresis, Gel, Two-Dimensional; Female; Humans; Infant; Leukemia, Myeloid, Acute; Male; Myelodysplastic Syndromes; Proteins; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
DOI:
10.1007/s00726-010-0718-9
