Τίτλος:
Antihyperlipidemic, Antihyperglycemic, and Liver Function Protection of Olea europaea var. Meski Stone and Seed Extracts: LC-ESI-HRMS-Based Composition Analysis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Methanol and methanol/water extracts of olive stones and seeds from Olea europaea var. meski were analyzed by reversed-phase high-performance liquid chromatography (HPLC) with diode array detection and mass spectrometry (LC-MS/MS). A total of 28 metabolites were identified; among them are hydroxycinnamic acid derivatives, phenolic alcohols, flavonoids and flavonoid glucosides, secoiridoids, and terpenes. All the extracts were screened for the inhibitory effect of key enzymes related to diabetes and obesity, such as α-amylase and lipase. An in vitro study revealed that Olea meski stone ethanol (MSE) and methanol (MSM) extracts and Olea meski seed ethanol (MSE1) and methanol (MSM1) extracts exert an inhibitory action against lipase and α-amylase. The most potent activity was observed in the StM extract with IC50 equal to 0.19 mg/ml against DPPH oxidation, 1.04 mg/ml against α-amylase, and 2.13 mg/ml against lipase. In HFFD rats, the findings indicated that the increase of body weight, LDL, TC, and glucose levels and then the decrease in HDL-C were significantly suppressed in the MSM-treated group than those in HFFD rats. Moreover, the MSM extract exhibited a prominent selective inhibitory effect against intestinal lipase and α-amylase activities. The MSM extract was also able to protect the liver-kidney functions efficiently, which was evidenced by biochemicals and histological studies. © 2021 Amina Ben Saad et al.
Συγγραφείς:
Ben Saad, A.
Tiss, M.
Keskes, H.
Chaari, A.
Sakavitsi, M.E.
Hamden, K.
Halabalaki, M.
Allouche, N.
Περιοδικό:
Journal of Diabetes Research
Λέξεις-κλειδιά:
9,12,13 trihydroxy octadeca 7 enoic acid; acarbose; acetic acid ethyl ester; acteoside; alcohol; amylase; antidiabetic agent; antilipemic agent; arachidic acid; ascorbic acid; atorvastatin; cholesterol; comselogoside; dihydro oleuropein; dihydroxyheneicosanoic acid; elenolic acid; glucose; high density lipoprotein cholesterol; hydroxyoctadecadienoic acid; hydroxyoleuropein; hydroxytyrosol; hydroxytyrosol acetate; hydroxytyrosol acyclodihydroelenolate; hydroxytyrosol hexoside; isoacteoside; ligstroside; liver protective agent; low density lipoprotein; luteolin; luteolin 7 o rutinoside; luteolin hexoside; maslinic acid; methanol; neo nuzhenide; nuzhenide; Olea europaea var. meski extract; oleacein; oleanolic acid; oleoside; oleoside 11 methyl ester; oleuropein; oleuropein aglycon; plant extract; plant medicinal product; salidroside; triacylglycerol lipase; unclassified drug; amylase; antidiabetic agent; antilipemic agent; phytochemical; plant extract, animal experiment; animal model; animal tissue; antidiabetic activity; Article; body weight; chemical composition; cholesterol blood level; controlled study; DPPH radical scavenging assay; electrospray mass spectrometry; endocarp; enzyme activity; enzyme inhibition; glucose blood level; glucose level; high fat/high fructose diet; hypolipidemic activity; IC50; in vitro study; lipid blood level; liquid chromatography-mass spectrometry; liver function; liver protection; male; mass spectrometry; nonhuman; obesity; Olea europaea var. meski; olive tree; oxidation; phytochemistry; plant seed; rat; reversed phase high performance liquid chromatography; wood; animal; chemistry; drug effect; electrospray mass spectrometry; high performance liquid chromatography; liver; metabolism; olive tree; physiology; Wistar rat, alpha-Amylases; Animals; Chromatography, High Pressure Liquid; Hypoglycemic Agents; Hypolipidemic Agents; Liver; Male; Olea; Phytochemicals; Plant Extracts; Rats; Rats, Wistar; Seeds; Spectrometry, Mass, Electrospray Ionization
DOI:
10.1155/2021/6659415
