Τίτλος:
PIM-1 is overexpressed at a high frequency in circulating tumor cells from metastatic castration- resistant prostate cancer patients
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
PIM-1 is an oncogene involved in cell cycle progression, cell growth, cell survival and therapy resistance, activated in many types of cancer, and is now considered as a very promising target for cancer therapy. We report for the first time that PIM-1 is overexpressed in circulating tumor cells (CTCs) from metastatic castration-resistant prostate cancer patients (mCRPC). We first developed and validated a highly sensitive RT-qPCR assay for quantification of PIM-1 transcripts. We further applied this assay to study PIM-1 expression in EpCAM(+) CTC fraction isolated from 64 peripheral blood samples of 50 mCRPC patients. CTC enumeration in all samples was performed using the FDA-cleared CellSearch® system. PIM-1 overexpression was detected in 24/64 (37.5%) cases, while in 20/24 (83.3%) cases that were positive for PIM-1 expression, at least one CTC/7.5 mL PB was detected in the CellSearch®. Our data indicate that PIM-1 overexpression is observed at high frequency in CTCs from mCRPC patients and this finding, in combination with androgen receptor splice variant 7 (AR-V7) expression in CTCs, suggest its potential role as a very promising target for cancer therapy. We strongly believe that PIM-1 overexpression in EpCAM(+) CTC fraction merits to be further evaluated and validated as a non-invasive circulating tumor biomarker in a large and well-defined patient cohort with mCRPC. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Συγγραφείς:
Markou, A.
Tzanikou, E.
Strati, A.
Zavridou, M.
Mastoraki, S.
Bournakis, E.
Lianidou, E.
Περιοδικό:
Blood cancer journal
Λέξεις-κλειδιά:
abiraterone; androgen receptor; docetaxel; enzalutamide; epithelial cell adhesion molecule; protein androgen receptor splice variant 7; protein kinase Pim 1; unclassified drug, Article; blood sampling; carcinogenesis; castration resistant prostate cancer; cell fractionation; cell isolation; circulating tumor cell; clinical article; cohort analysis; controlled study; gene overexpression; genetic transcription; human; human cell; human tissue; male; metastasis; molecularly targeted therapy; oncogene; PIM 1 gene; real time polymerase chain reaction
DOI:
10.3390/cancers12051188
