Τίτλος:
IGF-IEC expression is associated with advanced differentiated thyroid cancer
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aim: Recent knowledge implicates a differential expression of the insulin-like growth factor-I (IGF-I) mRNA splice variants (i.e., IGF-IEa, IGF-IEb and IGF-IEc) in cancerous tissues, implying possible specific roles of the encoded IGF-I protein isoforms in cancer biology. In particular, there is growing evidence that the IGF-IEc isoform may play a distinct biological role in various types of cancers. The present study investigated whether IGF-IEc expression is associated with a particular type of thyroid cancer. Materials and Methods: Formalin-fixed paraffin-embedded tissue specimens of different types of thyroid cancers from 92 patients were assessed for IGF-IEc expression by immunohistochemistry. In addition, thyroid cancer biopsies of different TNM staging histological types were evaluated for mRNA expression of the IGF-IEc transcript by real-time polymerase chain reaction (PCR). Results: From the total number of 92 samples, 2 were anaplastic, 10 medullary, 4 hyperplasias of C-cells, 11 follicular, 5 hurtle cell carcinomas, 2 poorly differentiated, 5 nodular hyperplasias, 1 lymphoma and 52 were papillary thyroid cancers. The age of cancer diagnosis or tumor size did not significantly affect the IGF-IEc expression. Among all types of cancers, IGF-IEc was expressed in papillary differentiated thyroid cancer. Its expression/localization was mainly cytoplasmic and significantly associated with TNM staging and the presence of muscular and capsule cancerous invasion (p<0.05). Similarly, a differential profile was revealed regarding the mRNA expression of the IGF-IEc transcript, that exhibited a higher expression in aggressive compared to the non-aggressive papillary cancers. Conclusion: IGF-IEc isoform expression in thyroid cancer is positively associated with more advanced stages of papillary thyroid cancer. © 2019 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Karagiannis, A.K.
Philippou, A.
Tseleni-Balafouta, S.
Zevolis, E.
Nakouti, T.
Tsopanomichalou-Gklotsou, M.
Psarras, V.
Koutsilieris, M.
Περιοδικό:
ANTICANCER RESEARCH
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
insulin like growth factor iEc; somatomedin C; unclassified drug; IGF1 protein, human; somatomedin C, adolescent; adult; advanced cancer; aged; Article; cancer staging; controlled study; differentiated thyroid cancer; human; human tissue; hurtle cell carcinoma; immunohistochemistry; lymphoma; major clinical study; mRNA expression level; nodular hyperplasia; priority journal; real time polymerase chain reaction; retrospective study; thyroid follicular carcinoma; thyroid hyperplasia; thyroid papillary carcinoma; tumor biopsy; tumor volume; alternative RNA splicing; cytoplasm; female; gene expression regulation; genetics; male; metabolism; middle aged; pathology; thyroid tumor; upregulation; young adult, Adolescent; Adult; Aged; Alternative Splicing; Cytoplasm; Female; Gene Expression Regulation, Neoplastic; Humans; Insulin-Like Growth Factor I; Male; Middle Aged; Neoplasm Staging; Retrospective Studies; Thyroid Cancer, Papillary; Thyroid Neoplasms; Tumor Burden; Up-Regulation; Young Adult
DOI:
10.21873/anticanres.13409
