Τίτλος:
Evolutionary variability of w-linked repetitive content in lacertid lizards
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Lacertid lizards are a widely radiated group of squamate reptiles with long-term stable ZZ/ZW sex chromosomes. Despite their family-wide homology of Z-specific gene content, previous cytogenetic studies revealed significant variability in the size, morphology, and heterochromatin distribution of their W chromosome. However, there is little evidence about the accumulation and distribution of repetitive content on lacertid chromosomes, especially on their W chromosome. In order to expand our knowledge of the evolution of sex chromosome repetitive content, we examined the topology of telomeric and microsatellite motifs that tend to often accumulate on the sex chromosomes of reptiles in the karyotypes of 15 species of lacertids by fluorescence in situ hybridization (FISH). The topology of the above-mentioned motifs was compared to the pattern of heterochromatin distribution, as revealed by C-banding. Our results show that the topologies of the examined motifs on the W chromosome do not seem to follow a strong phylogenetic signal, indicating independent and species-specific accumulations. In addition, the degeneration of the W chromosome can also affect the Z chromosome and potentially also other parts of the genome. Our study provides solid evidence that the repetitive content of the degenerated sex chromosomes is one of the most evolutionary dynamic parts of the genome. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Συγγραφείς:
Suwala, G.
Altmanová, M.
Mazzoleni, S.
Karameta, E.
Pafilis, P.
Kratochvíl, L.
Rovatsos, M.
Περιοδικό:
Genes and Nutrition
Λέξεις-κλειδιά:
animal experiment; article; C banding; fluorescence in situ hybridization; heterochromatin; karyotype; lizard; nonhuman; sex chromosome; sexual development; telomere; animal; chromosome; chromosome banding pattern; comparative study; female; fluorescence in situ hybridization; genetics; heterochromatin; karyotype; lizard; male; molecular evolution; nucleotide motif; nucleotide repeat; phylogeny; sex chromosome; species difference; telomere; ultrastructure, microsatellite DNA, Animals; Chromosome Banding; Chromosomes; Evolution, Molecular; Female; Heterochromatin; In Situ Hybridization, Fluorescence; Karyotype; Lizards; Male; Microsatellite Repeats; Nucleotide Motifs; Phylogeny; Repetitive Sequences, Nucleic Acid; Sex Chromosomes; Species Specificity; Telomere
DOI:
10.3390/genes11050531
