Τίτλος:
Detection of superoxide alterations induced by 5-fluorouracil on HeLa cells with a cell-based biosensor
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: In vitro cell culture monitoring can be used as an indicator of cellular oxidative stress for the assessment of different chemotherapy agents. Methods: A cell-based bioelectric biosensor was used to detect alterations in superoxide levels in the culture medium of HeLa cervical cancer cells after treatment with the chemotherapeutic agent 5-fluorouracil (5-FU). The cytotoxic effects of 5-fluorouracil on HeLa cells were assessed by the MTT proliferation assay, whereas oxidative damage and induction of apoptosis were measured fluorometrically by the mitochondria-targeted MitoSOX™ Red and caspase-3 activation assays, respectively. Results: The results of this study indicate that 5-FU differentially affects superoxide production and caspase-3 activation when applied in cytotoxic concentrations against HeLa cells, while superoxide accumulation is in accordance with mitochondrial superoxide levels. Our findings suggest that changes in superoxide concentration could be detected with the biosensor in a non-invasive and rapid manner, thus allowing a reliable estimation of oxidative damage due to cell apoptosis. Conclusions: These findings may be useful for facilitating future high throughput screening of different chemotherapeutic drugs with a cytotoxic principle based on free radical production. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Συγγραφείς:
Mavrikou, S.
Tsekouras, V.
Karageorgou, M.-A.
Moschopoulou, G.
Kintzios, S.
Περιοδικό:
BIOSENSORS-BASEL
Λέξεις-κλειδιά:
3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; caspase 3; fluorouracil; superoxide; antineoplastic antimetabolite; fluorouracil; superoxide, antineoplastic activity; apoptosis; Article; bioaccumulation; cell based biosensor; cell proliferation assay; cell viability; controlled study; drug efficacy; enzyme activation; enzyme assay; fluorometry; HeLa cell line; high throughput screening; human; human cell; in vitro study; intracellular signaling; MTT assay; non invasive procedure; oxidation reduction state; oxidative stress; cell proliferation; cell survival; chemistry; drug effect; drug screening; genetic procedures; HeLa cell line; metabolism; mitochondrion, Antimetabolites, Antineoplastic; Apoptosis; Biosensing Techniques; Cell Proliferation; Cell Survival; Drug Screening Assays, Antitumor; Fluorouracil; HeLa Cells; Humans; Mitochondria; Superoxides
