Τίτλος:
Magnoflorine—isolation and the anticancer potential against nci-h1299 lung, mda-mb-468 breast, t98g glioma, and te671 rhabdomyosarcoma cancer cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Magnoflorine (MGN) is a quaternary aporphine alkaloid that exhibits numerous therapeutic properties, including neuropsychopharmacological, anti-anxiety, immunomodulatory, anti-inflammatory, antioxidant, or antifungal activities. The aim of the present study was an investigation of the influence of MGN on viability, proliferation, induction of apoptosis, and cell cycle arrest in NCI-H1299 lung, MDA-MB-468 breast, T98G glioma, and TE671 rhabdomyosarcoma cancer cells. MGN was isolated from the roots of Berberis cretica L. by counter-current partition chromatography (CPC). Cell viability and proliferation assessments were performed by means of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and 5-bromo-2′-deoxyuridine (BrDU) assays, respectively. The induction of apoptosis and cell cycle progression was measured using fluorescence-activated cell sorting analysis. MGN in high doses inhibits proliferation, induces apoptosis, and inhibits cell cycle in S/G2 phases in a dose-dependent manner. MGN seems to be a promising anti-cancer compound in therapy of some types of lung, breast, glioma, and rhabdomyosarcoma cancers, for which current standard therapies are limited or have severe strong side effects. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Συγγραφείς:
Okon, E.
Kukula-Koch, W.
Halasa, M.
Jarzab, A.
Baran, M.
Dmoszynska-Graniczka, M.
Angelis, A.
Kalpoutzakis, E.
Guz, M.
Stepulak, A.
Wawruszak, A.
Περιοδικό:
Ancient Biomolecules
Λέξεις-κλειδιά:
3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; acetonitrile; antioxidant; berberine; beroline; caspase 3; caspase 9; magnoflorine; methanol; palmatine; penicillin derivative; plant extract; antineoplastic agent; aporphine derivative; magnoflorine; plant extract, antineoplastic activity; antiproliferative activity; apoptosis; Article; BrdU assay; breast cancer; cell cycle arrest; cell cycle G2 phase; cell cycle S phase; cell proliferation; cell viability; controlled study; cytotoxicity; electrospray; enzyme linked immunosorbent assay; flow cytometry; fluorescence activated cell sorting; glioma; high performance liquid chromatography; human; human cell; IC50; lung cancer; mass fragmentography; MDA-MB-468 cell line; MTT assay; T98G cell line; time of flight mass spectrometry; Berberis; breast tumor; cell cycle checkpoint; cell survival; chemistry; drug effect; female; glioma; isolation and purification; pathophysiology; rhabdomyosarcoma; tumor cell line, Antineoplastic Agents; Apoptosis; Aporphines; Berberis; Breast Neoplasms; Cell Cycle Checkpoints; Cell Line, Tumor; Cell Survival; Female; Glioma; Humans; Plant Extracts; Rhabdomyosarcoma
DOI:
10.3390/biom10111532
