Τίτλος:
Α 2-stage, nested-like nucleic acid amplification method (IsoPCR)for the highly sensitive detection of HPV16 and HPV18 DNA
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Molecular detection of HPV DNA is considered as the gold standard for the diagnosis of cervical disease. Although the molecular assays for the identification of HPV16 and HPV18 have helped identify cervical cancer incidents, they are restricted to specialized laboratories. Thus, we developed a novel 2-stage, nested-like nucleic acid amplification method, named IsoPCR, to amplify the E6 gene of HPV16 and HPV18 with high analytical sensitivity and specificity. The performance of IsoPCR was compared to that of conventional PCR and LAMP. The analytical sensitivity of IsoPCR (1 copy/test)was 10-fold higher than conventional PCR and 25-fold higher than conventional LAMP. IsoPCR displayed significant amplification specificity (100%)and efficiency, as well. In conclusion, IsoPCR is a highly sensitive and specific diagnostic tool and it is suitable for the detection of low copy number of viral DNA in clinical specimens, providing critical information to healthcare providers. © 2019 Elsevier Ltd
Συγγραφείς:
Daskou, M.
Tsakogiannis, D.
Dimitriou, T.G.
Manali, M.
Apti, C.
Amoutzias, G.D.
Mossialos, D.
Kottaridi, C.
Markoulatos, P.
Περιοδικό:
Molecular and Cellular Probes
Εκδότης:
INSTAP Academic Press
Λέξεις-κλειδιά:
virus DNA; DNA binding protein; E6 protein, Human papillomavirus type 16; E6 protein, Human papillomavirus type 18; oncoprotein; repressor protein; virus DNA, Article; genotype; health care personnel; Human papillomavirus type 16; Human papillomavirus type 18; loop mediated isothermal amplification; nonhuman; nucleic acid amplification; polymerase chain reaction; priority journal; sensitivity and specificity; comparative study; female; genetics; human; Human papillomavirus type 16; Human papillomavirus type 18; isolation and purification; molecular diagnosis; nucleic acid amplification; papillomavirus infection; procedures; uterine cervix tumor; virology, DNA, Viral; DNA-Binding Proteins; Female; Human papillomavirus 16; Human papillomavirus 18; Humans; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Oncogene Proteins, Viral; Papillomavirus Infections; Polymerase Chain Reaction; Repressor Proteins; Sensitivity and Specificity; Uterine Cervical Neoplasms
DOI:
10.1016/j.mcp.2019.03.003
