Τίτλος:
The effect of transfusion on immune responses in thalassemia
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Regular transfusions are the gold standard therapy for β-thalassemia and are often complicated by secondary-iron overload and alloimmunization. We assessed the frequency of regulatory T cells (Tregs) and the levels of cytokines implicated in Th-responses in 49 patients 33 TDT and 16 NTDT in order to investigate the contribution of transfusion and its complications on immune responses. Materials and methods: Tregs were characterized with flow cytometry. Soluble IL-4, IL-6, IL-10, IL-17A, and TGF-β1 were assessed by ELISA. Clinical data including alloimmunization, age of onset of transfusion splenectomy hepatitis B and C infection, iron overload assessment with MRI T2* (liver and heart) were recorded from the patients' files. Results: Tregs levels, IL-6, IL-10, TGFβ and serum ferritin were higher in the TDT compared to the NTDT group (all p < 0.05). There was no difference of Tregs and circulating cytokines in patients in correlation with the extend of iron overload (assessed by T2*liver), the type of chelator or the development of alloantibodies. Discussion: Tregs levels are higher in TDT patients compared to NTDT, a difference which could be ascribed to transfusion. Tregs levels and the cytokines analyzed may play little role in alloimmunization and are not impacted by the extend of iron overload. © 2020
Συγγραφείς:
Politou, M.
Komninaka, V.
Valsami, S.
Kapsimali, V.
Pouliakis, A.
Koutsouri, T.
Panayiotakopoulos, G.
Kotsianidis, I.
Voskaridou, E.
Περιοδικό:
BLOOD CELLS MOLECULES AND DISEASES
Εκδότης:
Academic Press Inc.
Λέξεις-κλειδιά:
alloantibody; ferritin; interleukin 10; interleukin 17; interleukin 4; interleukin 6; transforming growth factor beta1; cytokine, adult; aged; alloimmunization; Article; blood transfusion; cellular immunity; controlled study; female; human; immune response; iron overload; major clinical study; male; priority journal; protein blood level; regulatory T lymphocyte; thalassemia; blood; immunology; lymphocyte count; middle aged; thalassemia; very elderly, Adult; Aged; Aged, 80 and over; Blood Transfusion; Cytokines; Female; Humans; Lymphocyte Count; Male; Middle Aged; T-Lymphocytes, Regulatory; Thalassemia
DOI:
10.1016/j.bcmd.2020.102425
