Τίτλος:
Study on the admission levels of circulating cell-free DNA in patients with acute myocardial infarction using different quantification methods
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Circulating cell-free DNA (cf-DNA) is present in human biological fluids, mainly in plasma and serum, originating from cell death, a process that massively takes place during acute myocardial infarction (AMI). In the present study, cf-DNA was assessed by different quantification techniques, in order to determine its levels in patients admitted with AMI. A total of 130 subjects were included in the study: 80 ST elevation myocardial infarction (STEMI) patients and 50 healthy controls. Cf-DNA extracted from plasma was analyzed by: a) Qubit 3.0 with single (ss) and double (ds) stranded DNA assay kits, b) NanoDrop and c) quantitative PCR (qPCR). Cf-DNA levels were recorded elevated in AMI patients compared to those of healthy individuals. Specifically, Qubit 3.0 ss-DNA kit provided the highest cf-DNA concentration values for all the samples analyzed in comparison with ds-DNA assay kit and NanoDrop, approaching the values obtained by qPCR. Cf-DNA augments in massive cell death settings, including AMI, proposing that the quantification of its levels by novel methodologies could contribute to patient diagnosis and clinical management. © 2020, © 2020 Medisinsk Fysiologisk Forenings Forlag (MFFF).
Συγγραφείς:
Agiannitopoulos, K.
Samara, P.
Papadopoulou, E.
Tsamis, K.
Mertzanos, G.
Babalis, D.
Lamnissou, K.
Περιοδικό:
Scandinavian Journal of Clinical and Laboratory Investigation
Εκδότης:
Taylor and Francis Ltd.
Λέξεις-κλειδιά:
circulating cell free DNA; DNA; unclassified drug; biological marker; cell free nucleic acid; DNA, acute heart infarction; adult; blood sampling; cell death; controlled study; DNA determination; DNA extraction; hospital admission; human; major clinical study; middle aged; Note; priority journal; real time polymerase chain reaction; ST segment elevation myocardial infarction; aged; blood; case control study; female; genetics; male; patient selection; ST segment elevation myocardial infarction, Aged; Biomarkers; Case-Control Studies; Cell-Free Nucleic Acids; DNA; Female; Humans; Male; Middle Aged; Patient Admission; Patient Selection; Real-Time Polymerase Chain Reaction; ST Elevation Myocardial Infarction
DOI:
10.1080/00365513.2020.1729400
