Τίτλος:
Crocins: The active constituents of crocus sativus l. stigmas, exert significant cytotoxicity on tumor cells in vitro
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Tumors of the childhood are considered to be grave and devastating pa-thologies, with high mortality rates. Current therapeutic options like cytotoxic drugs and radiotherapy target both healthy and malignant cells, thus resulting in long-term neurological and intellectual sequelae and endocrinological disorders. Objectives: In this study, we focused on the anticancer potency of crocins, the main constituents of Crocus sativus L, stigmas. Crocins were first extracted using organic solvents from the dried stigmas and then were identified using the HPLC analysis. Materials and Methods: TE-671 cells were treated with the extract of crocins using a range of concentrations between 0.25-mg/ mL and 16 mg/mL. Viability of the cells was measured at 24h, 48h, 72h and 96h. In addition, we have examined the expression levels of the p53 gene using Real-Time Reverse Transcription PCR. Results: Results showed that crocins exerted significant cytotoxic and anti-proliferative effects in a concentration and time-dependent-manner on TE-671 cells. Furthermore, p53 manifested similar expression pattern as the anti-proliferative effect of crocin. Conclusion: Our data demonstrate that crocins could be a novel promising agent for the improvement of tumor treatment. © 2019 Bentham Science Publishers.
Συγγραφείς:
Hatziagapiou, K.
Kakouri, E.
Lambrou, G.I.
Koniari, E.
Kanakis, C.
Nikola, O.A.
Theodorakidou, M.
Bethanis, K.
Tarantilis, P.A.
Περιοδικό:
Current Cancer Therapy Reviews
Εκδότης:
Bentham Science Publishers
Λέξεις-κλειδιά:
alpha carotene; beta carotene; crocetin; crocin; Crocus sativus extract; gentiobiose; lycopene; protein p53; zeaxanthin, antineoplastic activity; antiproliferative activity; apoptosis; Article; cell proliferation; cell viability; controlled study; Crocus sativus; cytotoxicity; gene expression; high performance liquid chromatography; human; human cell; IC50; in vitro study; pistil stigma; priority journal; protein expression; protein expression level; real time reverse transcription polymerase chain reaction; resazurin assay; retention time (chromatography); TE 671 cell line; tumor cell; ultraviolet visible spectrophotometry
DOI:
10.2174/1573394714666181029120446
