Τίτλος:
Platelet depletion/transfusion as a lethal factor in a colitis-associated cancer mouse model
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aim: TLR-4 Knock-out (KO) mice are protected from colitis-associated cancer in the established AOM/DSS mouse model. The aim of this study was to assess whether the TLR4 KO mice would still be protected from carcinogenesis after platelet depletion and transfusion with TLR4 wild-type platelets. Materials and Methods: Thirty-two female C57BL6 mice were divided into 6 groups. Among the three groups that received Azoxymethane/Dextran Sulfate Sodium (AOM/DSS), one group included TLR4KO mice, which were depleted of their platelets and were then transfused with platelets from TLR4 wild-type mice. The other two groups included wild-type and TLR-4KO mice that only received AOM/DSS. Results: All 6 animals in the KO group that underwent platelet depletion/transfusion succumbed. Three of them died before the administration of DSS and three in the week following DSS administration. In contrast, mice in the other two groups experienced less weight loss and only 1 mouse died in each of them. Conclusion: Platelet depletion/transfusion was detrimental in TLR-4 transgenic mice that received AOM/DSS. © 2019 International Institute of Anticancer Research. All rights reserved.
Συγγραφείς:
Angelou, A.
Antoniou, E.
Pikouli, A.
Wang, J.
Ventin, M.
Buettner, S.
Faateh, M.
Theodoropoulos, G.
Zografos, G.C.
Theocharis, S.
Papalois, A.E.
Pikoulis, E.
Margonis, G.A.
Περιοδικό:
ANTICANCER RESEARCH
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
azoxymethane; dextran sulfate; toll like receptor 4; azoxymethane; dextran sulfate; Tlr4 protein, mouse; toll like receptor 4, animal experiment; animal model; animal tissue; Article; body weight loss; cancer prevention; colitis; colitis associated colorectal cancer; colon carcinogenesis; colorectal cancer; controlled study; death; dextran sulfate sodium-induced colitis; female; knockout mouse; lethality; loose feces; mouse; nonhuman; peritonitis; platelet depletion; priority journal; rectum hemorrhage; thrombocyte transfusion; thrombocytopenia; transgenic mouse; wild type mouse; animal; blood; carcinogenesis; chemically induced; colitis; colon tumor; complication; disease model; drug effect; genetics; human; metabolism; pathology; thrombocyte; thrombocyte transfusion, Animals; Azoxymethane; Blood Platelets; Carcinogenesis; Colitis; Colonic Neoplasms; Dextran Sulfate; Disease Models, Animal; Humans; Mice; Mice, Knockout; Platelet Transfusion; Toll-Like Receptor 4
DOI:
10.21873/anticanres.13362
