Τίτλος:
Characterization of more than 85% of cystic fibrosis alleles in the Greek population, including five novel mutations
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
To completely characterize the spectrum of mutations in the cystic fibrosis transmembrane conductance regulator gene in Greek cystic fibrosis (CF) patients, we screened 500 CF chromosomes by denaturing gradient gel electrophoresis followed by direct sequencing. We identified 48 mutations, accounting for 85.6% of CF chromosomes. They included eight novel mutations, three of which we have described before and five (E822X, Y247X, 2752-26A→G, 31S2delT, and 2751+T→A), which are described in this report. The detection of such a high proportion of Greek CF mutations is important for improving prenatal and genetic diagnosis of CF in Greece.
Συγγραφείς:
Tzetis, M.
Kanavakis, E.
Antoniadi, T.
Doudounakis, S.
Adam, G.
Kattamis, C.
Περιοδικό:
Human Genetics
Λέξεις-κλειδιά:
transmembrane conductance regulator, adult; aged; allele; article; child; controlled study; cystic fibrosis; denaturing gradient gel electrophoresis; female; gene mutation; gene sequence; Greece; human; major clinical study; male; prenatal diagnosis; priority journal
DOI:
10.1007/s004390050324
