Τίτλος:
Hypoxaemic reperfusion ameliorates the histopathological changes in the
pig brain after a severe global cerebral ischaemic insult
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background and purpose: We have recently shown that hypoxaemic
reperfusion, after an ischaemic brain insult, improves neurological
outcome and decreases lipid peroxidation. In the present study, we
investigated the effect of hypoxaemic reperfusion on brain
histopathological changes.
Methods: Sixteen pigs subjected to a 10-min global cerebral ischaemia
were either hypoxaemically (PaO2 approximate to 35 mmHg, hypoxaemic
reperfusion (HR) group, n = 8) or hyperoxaemically (PaO2 > 300 mmHg,
control (C) group, n = 8) reperfused. The brains were removed 24 h after
reperfusion and six neuropathological abnormalities were evaluated
blindly and scored semi-quantitatively (0: normal to 3: severe injury)
on eight representative regions of the brain. The overall cumulative
score of the abnormalities and their regional prevalence, as well as the
neurological outcome, were compared between the two groups.
Results: The neuronal degeneration, assessed in terms of cumulative
score (P = 0.002) and regional prevalence (P = 0.025 to P = 0.041), was
lower in the HR group than in the C group. Spongy degeneration attained
statistically significant difference only in cerebellum (P = 0.002) and
inflammation only in hippocampus (P = 0.046) but the difference in the
cumulative score of these abnormalities was not statistically
significant. The difference of the three neurological assessments over
time was statistically significant between the two groups, i.e. after
resuscitation (P = 0.001), at 8 h (P = 0.006) and at 24 h (P = 0.001)
after reperfusion.
Conclusions: Hypoxaemic reperfusion during resuscitation from a severe
global ischaemic cerebral insult is associated with statistically
significantly fewer histopathological changes of the brain than in
controls. This is associated with a superior neurological outcome.
Συγγραφείς:
Douzinas, EE
Patsouris, E
Kypriades, EM
Makris, DJ and
Andrianakis, I
Korkolopoulou, P
Boursinos, V
Papalois, A and
Sotiropoulou, C
Davaris, P
Roussos, C
Περιοδικό:
Intensive Care Medicine Experimental
Λέξεις-κλειδιά:
hypoxaemic reperfusion; reactive oxygen species; overall performance
category; brain histopathology; postanoxic encephalopathy
DOI:
10.1007/s001340100932
