Τίτλος:
Molecular analysis of transferrin receptor mRNA expression in acute
myeloid leukaemia
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Transferrin receptor (TfR, CD71) is an integral membrane glycoprotein
that mediates cellular uptake of iron. In most tissues, TfR expression
is correlated positively with proliferation and regulated at the
post-transcriptional level. The available data regarding the pattern of
TfR gene expression in haematological malignancies are very Limited. In
the present study, we evaluated TfR gene expression at the molecular
level in bone marrow (BM) samples of 44 patients with de novo acute
myeloid leukaemia (AXIL) at diagnosis with BM blasts > 85%. TfR mRNA
levels were determined by densitometric analysis of quantitative reverse
transcription polymerase chain reaction products corresponding to TfR
exons 15-17. Each sample was tested in at least two independent
experiments. In 13/44 patients, TfR messages were not detected (this is
probably an underestimate as some positive results may be attributed to
residual normal erythroid cells present in the samples). In 17/44, TfR
mRNA levels were low-intermediate, and were high in the remaining
patients (14/44). TfR mRNA positivity was significantly associated with
older age. No statistically significant correlations were found either
with specific French-American-British (FAB) subtypes or attainment of
complete remission, incidence of relapse and survival (after adjusting
accordingly for age and FAB subtype). The absence of TfR mRNA
transcripts in a significant minority of cases suggests that alternative
mechanisms of iron uptake may function in AML blast cells.
Συγγραφείς:
Kollia, P
Stavroyianni, N
Stamatopoulos, K
Zoi, K and
Viniou, N
Mantzourani, M
Noguchi, CT
Paterakis, G and
Abazis, D
Pangalos, C
Loukopoulos, D
Yataganas, X
Περιοδικό:
British Journal of Haematology
Λέξεις-κλειδιά:
transferrin receptor; mRNA; acute myeloid leukaemia
DOI:
10.1046/j.1365-2141.2001.03065.x
