Τίτλος:
Mutation detection of the human glucocorticoid receptor alpha gene area
coding for the hormone-binding domain by denaturing gradient gel
electrophoresis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Mutations in the hormone-binding domain of the human glucocorticoid
receptor alpha (hGRalpha) gene have been detected in a variety of
glucocorticoid resistance syndromes. Using the denaturing gradient gel
electrophoresis technique, we developed a sensitive method for the
detection of alterations in the gene area coding for the whole
hormone-binding domain and part of the DNA-binding domain of the
hGRalpha. This method can be applied for screening of glucocorticoid
receptor gene alterations in glucocorticoid-dependent diseases. (C) 2002
Elsevier Science B.V. All tights reserved.
Συγγραφείς:
Tsolakidou, AF
Coulocheri, SA
Sekeris, CE
Moutsatsou, P
Περιοδικό:
Journal of Biochemical and Biophysical Methods
Εκδότης:
ELSEVIER SCIENCE BV
Λέξεις-κλειδιά:
DGGE; glucocorticoid receptor; mutation detection
DOI:
10.1016/S0165-022X(02)00012-X
