Τίτλος:
Hepatic stimulator substance activity in animal model of fulminant
hepatic failure and encephalopathy
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Hepatic stimulator substance (HSS) is a known liver-specific but
species-nonspecific growth factor. In the present study we examined the
activity of the endogenously produced HSS in an established experimental
model of fulminant hepatic failure (FHF) and encephalopathy, induced by
repeated injections of thioacetamide (TAA). FHF was induced by three
consecutive intraperitoneal injections of TAA (400 mg/kg body weight) in
rats, at time intervals of 24 hr. The animals were killed at 0, 6, 12,
or 18 hr following the last injection of TAA. The rate of tritiated
thymidine incorporation into hepatic DNA, the enzymatic activity of
liver thymidine kinase (EC 2.7.1.21), and the assessment of mitotic
index in hepatocytes were used to estimate liver regeneration. HSS
extract obtained from the livers of TAA-treated rats, sacrificed at the
above-mentioned time points was tested for its activity. Increased HSS
activity was noted in all TAA-treated animals, presenting a peak at 12
hr following the third TAA dose, suggesting active participation of this
growth factor in hepatocyte replication in this animal model of FHF and
encephalopathy. It may also be suggested that up-regulation of HSS
activity could be used in future as a therapeutic approach in FHF.
Συγγραφείς:
Margeli, AP
Manolis, E
Skaltsas, SN
Tsarpalis, KS and
Mykoniatis, MG
Theocharis, SE
Περιοδικό:
Digestive Diseases and Sciences
Εκδότης:
KLUWER ACADEMIC/PLENUM PUBL
Λέξεις-κλειδιά:
hepatic stimulator substance; fulminant hepatic failure; hepatic
encephalopathy; thioacetamide
DOI:
10.1023/A:1020166706833
