Περίληψη:
Matrix metalloproteinases constitute one of the major extracellular
matrix degrading enzymic families implicated in cancer development.
Stromelysin-3 in particular, a member of the matrix metalloproteinases
belonging to the stromelysins’ subgroup, seems to be closely related to
invasiveness and tumor progression. In this study, we proceeded to the
evaluation of stromelysin-3 protein’s expression in paraffin sections of
133 cases of invasive breast carcinomas and statistically estimated its
relations with known clinicopathological prognostic parameters and
patients’ survival, proliferation markers Ki-67 and TopoIIalpha and the
antiapoptotic protein bcl-2. Presence of stromelysin-3 was
immunodetected, in the 73% of our cases, in stromal cells (65%) and in
epithelial tumor cells (26.26%). Stromelysin-3 epithelial positivity
presented statistically significant correlations with TopoIIalpha and
Ki-67 proliferation indices (P = .042 and P = .031, respectively) and
worse disease outcome through multivariate statistics (P = .014).
Stromelysin-3 fibroblastic expression was significantly associated with
nuclear grade (P = .024), ductal histological type (P = .037),
TopoIIalpha (P = .001) and Ki-67 (P = .019), inversely with bcl-2
protein (P = .027) and with adverse overall survival through univariate
analysis (P = .017). The subgroup of patients with stromelysin-3
co-expression in stromal and malignant epithelial cells showed
statistically significant associations with Ki-67 and TopoIIalpha (P =
.019, P < .0001, respectively), an inverse one with bcl-2 protein (P =
.027) and furthermore with impaired survival (P = .002) through
multivariate analysis. In conclusion, stromelysin-3 protein expression
correlated with proliferation indices TopoII alpha and Ki-67 and the
anti-apoptotic protein bcl-2, data confirming stromelysin-3’s
contribution to breast cancer progression. Moreover its expression was
shown to have a direct negative effect on patients’ survival, especially
in the subgroup of patients with simultaneous epithelial and stromal
expression.
Συγγραφείς:
Nakopoulou, L
Panayotopoulou, EG
Giannopoulou, I
Alexandrou,
P
Katsarou, S
Athanassiadou, P
Keramopoulos, A
