Τίτλος:
Molecular sub-grouping of enterovirus reference and wild type strains
into distinct genetic clusters using a simple RFLP assay
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
RFLP analysis and sequencing of RT-PCR amplicons in previous studies
revealed the existence of intra-serotypic variability in the 5’-UTR of
human enteroviruses, complicating the use of this method to serotype
isolates. During the present study, the available sequences of many
enterovirus reference and wild type strains were analysed in an attempt
to discover restriction sites that would rapidly and reliably aid the
classification of human enteroviruses into specific sub-groups on the
basis of their 5’-UTR for diagnostic and/or epidemiological purposes.
Despite intratypic genetic variability in the 5-UTR, the results of the
sequence analysis, as well as data from the RFLP analysis of 61
enterovirus reference strains from 60 different serotypes and 123
clinical isolates showed that one restriction endonuclease, HpaII, may
contribute to a reliable sub-classification of CAVs and the rest of
enteroviruses, on the basis of 5-UTR, into five genetic groups, which
could be particularly useful in clinical and epidemiological studies.
Although more sequence data from enterovirus reference and wild type
strains may be required for the elaboration of a precise molecular
identification system, the more possible genotypic classification into
distinct clusters, as shown with the restriction enzyme HpaII, and the
determination of the biological significance of this grouping
(pathogenesis, epidemiology) might constitute an alternative means of
enterovirus identification against conventional classification into
distinct serotypes. (C) 2003 Elsevier Science Ltd. All rights reserved.
Συγγραφείς:
Siafakas, N
Markoulatos, P
Vlachos, C
Stanway, G and
Tzanakaki, G
Kourea-Kremastinou, J
Περιοδικό:
Molecular and Cellular Probes
Εκδότης:
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Λέξεις-κλειδιά:
enteroviruses; 5 ‘-UTR; RT-PCR; restriction fragment length
polymorphism; genetic clusters
DOI:
10.1016/S0890-8508(03)00029-X
