Τίτλος:
Donor lung pretreatment with surfactant in experimental transplantation
preserves graft hemodynamics and alveolar morphology
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
In experimental lung transplantation, the reduction of endogenous
surfactant properties occurs after graft preservation and transplant
reperfusion. The aim of this study was to evaluate the efficacy of donor
lung pretreatment with exogenous surfactant on graft damage after
ischemia and reperfusion. Fourteen (control group A, n = 8; study group
B, n = 6) young female white pigs (mean weight 27 +/- 3.5 kg) were used
in a newly developed autotransplantation model with in situ cold
ischemia. In study group B, before thoracotomy, 1.5 ml/kg surfactant
apoprotein-A-free surfactant was administrated into the left main
bronchus via flexible bronchoscopy. Belzer UW solution was used for lung
preservation. Cold ischemia was achieved for 3 hr with interlobar lung
parenchyma temperature at 8 +/- 1.3degreesC, and central temperature
maintained at 37.20 +/- 0.5degreesC. Animals were sacrificed after 3 hr
of graft reperfusion. At the end of reperfusion, pulmonary vascular
resistance index (was 447.80 dyn/sec.cm(5).m(2) (+/-66.8) in group A vs
249.51 in group B (P < 0.001) and serum nitric oxide was adequately
preserved. The mean alveolar surface area estimated by computerized
morphometry was 5280.84 (4991.1) mum(2) (group A) vs 3997.89 (3284.70)
mum(2) (group B; P < 0.005). Histology revealed milder macrophage and
lymphocyte infiltration in group B at the end of reperfusion.
Pretreatment of donor lung with an surfactant apoprotein-A -free
surfactant agent appears to be beneficial in terms of maintaining serum
NO and reducing hemodynamic disturbances. Furthermore, alveolar
histology and stereomorphology are better preserved.
Συγγραφείς:
Koletsis, E
Chatzimichalis, A
Fotopoulos, V
Kokkinis, K and
Papadimitriou, E
Tiniakos, D
Marinos, E
Bellenis, I and
Dougenis, D
Περιοδικό:
Experimental Biology and Medicine
Εκδότης:
SAGE Publications Ltd
Λέξεις-κλειδιά:
lung transplantation; surfactant; nitric oxide; SP-A; experimental
reperfusion injury
DOI:
10.1177/15353702-0322805-23
