Τίτλος:
Assessment of a new immunocytochemical technique in HbF-cell counting by
a novel objective evaluation method
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A recently developed immunocytochemical technique in HbF-cell counting
was assessed by an objective evaluation method. The basic principle of
this method is the preparation of aliquots with predetermined HbF-cell
(target) values. These aliquots serve as control samples to standardize
the HbF-cell measurements by the new immunocytochemical technique, which
uses the StreptABComplex/AP staining procedure (SAP) and visualization
under white light. Immunofluorescence optical counts (IF) were performed
in parallel with the new technique. A trend of inaccuracy was observed
in low target values for both methods. As the level of target values
increased, deviations became insignificant (relative accuracy < 8%)
with SAP having slightly better results. Linear regression data of the
estimated %HbF-cell rates by the two methods versus the target values
were very satisfactory for both methods with SAP being slightly better.
SAP seems to provide an accurate and reliable alternative for HbF-cell
estimation comparable with the classical IF optical count.
Συγγραφείς:
Tsantes, A
Androutsos, G
Bonovas, S
Vogiatzi, D
Meletis,
I
Stamou, E
Stamoulakatou, A
Paterakis, G
Περιοδικό:
British Journal of Haematology
Εκδότης:
Wiley-Blackwell Publishing Ltd
Λέξεις-κλειδιά:
HbF cell; immunocytochemical technique; light microscopy; evaluation
method; target values
DOI:
10.1046/j.1365-2141.2003.04717.x
