Τίτλος:
Proteomic analysis of normal and cancer cervical cell lines reveals deregulation of cytoskeleton-associated proteins
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Both HPV-positive and -negative cervical cancers are primarily associated with features of cell cycle and cytoskeletal disruption; however, the actual biological processes affected remain elusive. To this end, we systematically characterized the intracellular proteomic profiles of four distinct and informative cervical cell lines. Materials and Methods: Cell extracts from a normal cervical (HCK1T) and three cervical cancer cell lines, one HPVnegative (C33A), and two HPV-positive, SiHa (HPV16+) and HeLa (HPV18+), were analyzed by 2-dimensional electrophoresis and differentially expressed proteins were identified by MALDI-TOF mass spectrometry, while differential expression was confirmed by western blot analysis. Results: In total, 133 proteins were found differentially expressed between the normal and the cervical cancer lines. Bioinformatics analysis revealed the actin cytoskeleton signaling pathway to be significantly affected, while up-regulation of cofilin-1, an actin depolymerizing factor, was documented and further validated by western blotting. Furthermore, two-way comparisons among the four cell lines, revealed a set of 18 informative differentially expressed proteins. Conclusion: These novel identified proteins provide the impetus for further functional studies to dissect the mechanisms operating in the two distinct pathways of cervical carcinogenesis.
Συγγραφείς:
Pappá, K.I.
Lygirou, V.
Kontostathi, G.
Zoidakis, J.
Makridakis, M.
Vougas, K.
Daskalakis, G.
Polyzos, A.
Anagnou, N.P.
Περιοδικό:
Cancer Genomics and Proteomics
Εκδότης:
International Institute of Anticancer Research
Λέξεις-κλειδιά:
actin; actin depolymerizing factor; cell extract; cofilin 1; cytoskeleton protein; cytoskeleton protein, actin filament; Article; bioinformatics; carcinogenesis; cervical cancer cell line; controlled study; female; HeLa cell line; human; human cell; Human papillomavirus type 16; Human papillomavirus type 18; in vitro study; keratinocyte; matrix assisted laser desorption ionization time of flight mass spectrometry; protein analysis; protein expression; protein function; proteomics; signal transduction; two dimensional electrophoresis; upregulation; uterine cervix; virus strain; Wart virus; Western blotting; matrix-assisted laser desorption-ionization mass spectrometry; metabolism; pathology; tumor cell line; two dimensional gel electrophoresis; uterine cervix tumor, Cell Line, Tumor; Cytoskeletal Proteins; Electrophoresis, Gel, Two-Dimensional; Female; Humans; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Uterine Cervical Neoplasms
