Τίτλος:
Effect of steviol, steviol glycosides and stevia extract on glucocorticoid receptor signaling in normal and cancer blood cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The use of steviol glycosides as non-caloric sweeteners has proven to be beneficial for patients with type 2 diabetes mellitus (T2D), obesity, and metabolic syndrome. However, recent data demonstrate that steviol and stevioside might act as glucocorticoid receptor (GR) agonists and thus correlate with adverse effects on metabolism. Herein, we evaluated the impact of steviol, steviol glycosides, and a Greek-derived stevia extract on a number of key steps of GR signaling cascade in peripheral blood mononuclear cells (PBMCs) and in Jurkat leukemia cells. Our results revealed that none of the tested compounds altered the expression of primary GR-target genes (GILZ, FKPB5), GR protein levels or GR subcellular localization in PBMCs; those compounds increased GILZ and FKPB5 mRNA levels as well as GRE-mediated luciferase activity, inducing in parallel GR nuclear translocation in Jurkat cells. The GR-modulatory activity demonstrated by stevia-compounds in Jurkat cells but not in PBMCs may be due to a cell-type specific effect. © 2017 Elsevier B.V.
Συγγραφείς:
Panagiotou, C.
Mihailidou, C.
Brauhli, G.
Katsarou, O.
Moutsatsou, P.
Περιοδικό:
Molecular and Cellular Endocrinology
Εκδότης:
Elsevier Ireland Ltd
Λέξεις-κλειδιά:
glucocorticoid receptor; luciferase; messenger RNA; plant extract; plant glycoside; Stevia extract; steviol; steviol glycoside; unclassified drug; corticotropin; dexamethasone; fk 506 binding protein; glucocorticoid receptor; glucoside; hydrocortisone; kaurane derivative; luciferase; messenger RNA; plant extract; rebaudioside A; steviol; stevioside; tacrolimus binding protein 5; transcription factor; TSC22D3 protein, human, Article; biochemical composition; cancer blood cell; cancer cell; cellular distribution; controlled study; drug structure; enzyme activity; FKPB5 gene; gene expression; GILZ gene; human; human cell; intracellular signaling; Jurkat cell line; oncogene; peripheral blood mononuclear cell; priority journal; protein localization; Stevia; Western blotting; blood; cell nucleus; cell survival; chemistry; DNA responsive element; drug effect; gene expression regulation; genetics; metabolism; mononuclear cell; neoplasm; signal transduction, Adrenocorticotropic Hormone; Cell Nucleus; Cell Survival; Dexamethasone; Diterpenes, Kaurane; Gene Expression Regulation; Glucosides; Humans; Hydrocortisone; Jurkat Cells; Leukocytes, Mononuclear; Luciferases; Neoplasms; Plant Extracts; Receptors, Glucocorticoid; Response Elements; RNA, Messenger; Signal Transduction; Stevia; Tacrolimus Binding Proteins; Transcription Factors
DOI:
10.1016/j.mce.2017.07.023
