Τίτλος:
miR-26a Mediates Adipogenesis of Amniotic Fluid Mesenchymal Stem/Stromal Cells via PTEN, Cyclin E1, and CDK6
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Recent findings indicate that microRNAs (miRNAs) are critical for the regulatory network of adipogenesis in human mesenchymal stem/stromal cells (MSCs). Fetal MSCs derived from amniotic fluid (AF-MSCs) represent a population of multipotent stem cells characterized by a wide range of differentiation properties that can be applied in cell-based therapies. In this study, miRNA microarray analysis was performed to assess miRNA expression in terminal differentiated AF-MSCs into adipocyte-like cells (AL cells). MiR-26a was identified in high expression levels in AL cells indicating a critical role in the process of adipogenesis. Overexpression of miR-26a in AF-MSCs led to significant induction of their adipogenic differentiation properties that were altered after miR-26a inhibition. We have demonstrated that miR-26a regulates adipogenesis through direct inhibition of PTEN, which in turn promotes activation of Akt pathway. Also, miR-26a modulates cell cycle during adipogenesis by interacting with Cyclin E1 and CDK6. These results point to the regulatory role of miR-26a and its target genes PTEN, Cyclin E1, and CDK6 in adipogenic differentiation of AF-MSCs, providing a basis for understanding the mechanisms of fat cell development and obesity.
Συγγραφείς:
Trohatou, O.
Zagoura, D.
Orfanos, N.K.
Pappa, K.I.
Marinos, E.
Anagnou, N.P.
Roubelakis, M.G.
Περιοδικό:
Stem Cells and Development
Λέξεις-κλειδιά:
CCNE1 protein, human; CDK6 protein, human; cyclin dependent kinase 6; cyclin E; microRNA; MIRN26A microRNA, human; oncoprotein; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; PTEN protein, human, adipocyte; adipogenesis; adipose tissue; amnion fluid; bone development; cell culture; cytology; genetics; human; mesenchymal stroma cell; metabolism; multipotent stem cell, Adipocytes; Adipogenesis; Adipose Tissue; Amniotic Fluid; Cells, Cultured; Cyclin E; Cyclin-Dependent Kinase 6; Humans; Mesenchymal Stromal Cells; MicroRNAs; Multipotent Stem Cells; Oncogene Proteins; Osteogenesis; PTEN Phosphohydrolase
DOI:
10.1089/scd.2016.0203
