Τίτλος:
MiR-224 overexpression is a strong and independent prognosticator of short-term relapse and poor overall survival in colorectal adenocarcinoma
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Colorectal adenocarcinoma constitutes the most frequent form of colorectal cancer and a serious cause of cancer-related deaths. The expression of multiple miRNAs, including miR-224, is deregulated in colorectal adenocarcinoma. The aim of this study was the investigation of the prognostic value of miR-224 in colorectal adenocarcinoma. For this purpose, total RNA was isolated from 115 colorectal adenocarcinomas and 66 adjacent non-cancer mucosae. Total RNA (2 μg) was polyadenylated and reverse transcribed. A quantitative PCR method based on SYBR-Green chemistry was developed and applied for the quantification of miR-224 levels, followed by extensive biostatistical analysis. miR-224 levels in malignant colorectal adenocarcinomas ranged between 1.81 and 187.75 RQU (miR-224 copies/1, 000 SNORD48 copies) with a median of 34.27, and were significantly elevated, compared to miR-224 levels in adjacent non-cancer mucosae (p<0.001). Enhanced miR-224 expression constitutes a rather strong prognosticator in colorectal adenocarcinoma, predicting short-term relapse and poor overall survival in these patients (p=0.012 and p=0.005, respectively), independent of established clinicopathological parameters. In conclusion, miR-224 is significantly upregulated in malignant colorectal tumors compared to adjacent non-cancer mucosae, and its enhanced expression constitutes an independent predictor of short-term relapse and poor overall survival in colorectal adenocarcinoma patients.
Συγγραφείς:
Adamopoulos, P.G.
Kontos, C.K.
Rapti, S.-M.
Papadopoulos, I.N.
Scorilas, A.
Περιοδικό:
INTERNATIONAL JOURNAL OF ONCOLOGY
Εκδότης:
Spandidos Publications
Λέξεις-κλειδιά:
microRNA; microRNA 224; polyadenylated RNA; unclassified drug; microRNA; MIRN224 microRNA, human, adult; aged; Article; cancer prognosis; cancer recurrence; cancer size; cancer staging; cancer survival; colorectal carcinoma; controlled study; distant metastasis; female; follow up; gene overexpression; human; human cell; human tissue; major clinical study; male; overall survival; real time polymerase chain reaction; tumor invasion; adenocarcinoma; biosynthesis; colorectal tumor; gene expression regulation; genetics; Kaplan Meier method; lymph node metastasis; middle aged; pathology; prognosis; recurrent disease; tumor recurrence; very elderly, Adenocarcinoma; Adenocarcinoma; Adult; Adult; Aged; Aged; Aged, 80 and over; Aged, 80 and over; Colorectal Neoplasms; Colorectal Neoplasms; Female; Female; Gene Expression Regulation, Neoplastic; Gene Expression Regulation, Neoplastic; Humans; Humans; Kaplan-Meier Estimate; Kaplan-Meier Estimate; Lymphatic Metastasis; Lymphatic Metastasis; Male; Male; MicroRNAs; MicroRNAs; Middle Aged; Middle Aged; Neoplasm Recurrence, Local; Neoplasm Recurrence, Local; Prognosis; Prognosis; Recurrence; Recurrence
DOI:
10.3892/ijo.2014.2775
