Τίτλος:
Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
In a previous study, we demonstrated that a grape pomace extract (GPE) exerted antioxidant activity in endothelial (EA.hy926) and muscle (C2C12) cells through an increase in glutathione (GSH) levels. In the present study, in order to elucidate the mechanisms responsible for the antioxidant activity of GPE, its effects on the expression of critical antioxidant enzymes, such as catalase (CAT), superoxide dismutase (SOD)1, heme oxygenase 1 (HO-1) and gamma-glutamylcysteine synthetase (GCS) were assessed in EA.hy926 and C2C12 cells. Moreover, the effects of GPE on CAT, SOD and glutathione S-transferase (GST) enzymatic activity were evaluated. For this purpose, the C2C12 and EA.hy926 cells were treated with GPE at low and non-cytotoxic concentrations (2.5 and 10 μg/ml for the C2C12 cells; 0.068 and 0.250 μg/ml for the EA.hy926 cells) for 3, 6, 12, 18 and 24 h. Following incubation, enzymatic expression and activity were assessed. The results revealed that treatment with GPE significantly increased GCS levels and GST activity in both the C2C12 and EA.hy926 cells. However, GPE significantly decreased CAT levels and activity, but only in the muscle cells, while it had no effect on CAT levels and activity in the endothelial cells. Moreover, treatment with GPE had no effect on HO-1 and SOD expression and activity in both cell lines. Therefore, the present results provide further evidence of the crucial role of GSH systems in the antioxidant effects exerted by GPE. Thus, GPE may prove to be effective for use as a food supplement for the treatment of oxidative stress-induced pathological conditions of the cardiovascular and skeletal muscle systems, particularly those associated with low GSH levels.
Συγγραφείς:
Goutzourelas, N.
Stagos, D.
Housmekeridou, A.
Karapouliou, C.
Kerasioti, E.
Aligiannis, N.
Skaltsounis, A.L.
Spandidos, D.A.
Tsatsakis, A.M.
Kouretas, D.
Περιοδικό:
International Journal of Molecular Medicine
Εκδότης:
Spandidos Publications
Λέξεις-κλειδιά:
antioxidant; catalase; glutamate cysteine ligase; glutathione transferase; Grape pomace extract; heme oxygenase 1; plant extract; superoxide dismutase; unclassified drug; antioxidant; catalase; copper zinc superoxide dismutase; glutamate cysteine ligase; glutathione; glutathione transferase; heme oxygenase 1; plant extract; SOD1 protein, human; Sod1 protein, mouse; superoxide dismutase, antioxidant activity; Article; controlled study; endothelium cell; enzyme activity; grape; muscle cell; priority journal; protein expression; Western blotting; animal; cell line; chemistry; drug effects; endothelium cell; enzyme activation; human; metabolism; mouse; muscle cell; oxidative stress; Vitis, Animals; Antioxidants; Catalase; Cell Line; Endothelial Cells; Enzyme Activation; Glutamate-Cysteine Ligase; Glutathione; Glutathione Transferase; Heme Oxygenase-1; Humans; Mice; Muscle Cells; Oxidative Stress; Plant Extracts; Superoxide Dismutase; Superoxide Dismutase-1; Vitis
DOI:
10.3892/ijmm.2015.2246
