Τίτλος:
The anti-inflammatory effects of exercise training promote atherosclerotic plaque stabilization in apolipoprotein E knockout mice with diabetic atherosclerosis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Physical exercise is the cornerstone of cardiovascular disease treatment. The present study investigated whether exercise training affects atherosclerotic plaque composition through the modification of inflammatoryrelated pathways in apolipoprotein E knockout (apoE-/-) mice with diabetic atherosclerosis. Forty-five male apoE-/mice were randomized into three equivalent (n=15) groups: control (CO), sedentary (SED), and exercise (EX). Diabetes was induced by streptozotocin administration. High-fat diet was administered to all groups for 12 weeks. Afterwards, CO mice were euthanatized, while the sedentary and exercise groups continued high-fat diet for 6 additional weeks. Exercising mice followed an exercise program on motorizedtreadmill (5 times/week, 60 min/session). Then, blood samples and atherosclerotic plaques in the aortic root were examined. A considerable (P<0.001) regression of the atherosclerotic lesions was observed in the exercise group (180.339±75.613×103μm2) compared to the control (325.485±72.302 ×103 μm2) and sedentary (340.188±159.108×103μm2) groups. We found decreased macrophages, matrix metalloproteinase-2 (MMP-2), MMP-3, MMP-8 and interleukin-6 (IL-6) concentrations (P<0.05) in the atherosclerotic plaques of the exercise group. Compared to both control and sedentary groups, exercise training significantly increased collagen (P<0.05), elastin (P<0.001), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) (P<0.001) content in the atherosclerotic plaques. Those effects paralleled with increased fibrous cap thickness and less internal elastic lamina ruptures after exercise training (P<0.05), while body-weight and lipid parameters did not significantly change. Plasma MMP-2 and MMP-3 concentrations in atherosclerotic tissues followed a similar trend. From our study we can conclude that exercise training reduces and stabilizes atherosclerotic lesions in apoE-/-mice with diabetic atherosclerosis. A favorable modification of the inflammatory regulators seems to explain those beneficial effects. © N.P.E. Kadoglou et al., 2013 Licensee PAGEPress, Italy.
Συγγραφείς:
Kadoglou, N.P.E.
Moustardas, P.
Kapelouzo, A.
Katsimpoulas, M.
Giagini, A.
Dede, E.
Kostomitsopoulos, N.
Karayannacos, P.E.
Kostakis, A.
Liapis, C.D.
Περιοδικό:
European Journal of Histochemistry
Εκδότης:
Luigi Ponzio e figlio Editori
