Τίτλος:
Peripheral blood lymphocytes from patients with bipolar disorder demonstrate apoptosis and differential regulation of advanced glycation end products and s100b
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: This study addresses the expression of the glycosylated proteins known as advanced glycation end products (AGEs), the calcium binding protein S100B and the apoptotic parameters cytochome c and caspase-3 activity in peripheral lymphocyte cytosolic extracts from a sample of bipolar disorder (BD) patients and healthy (control) subjects. Methods: Cross-sectional study of 35 patients with a clinical diagnosis of bipolar disease (10 euthymic, 12 depressed, 13 manic) and 10 healthy control subjects. Lymphocytes were used as a surrogate model in BD diagnosis and treatment. AGEs and S100B in lymphocyte cell extracts were measured by commercially available enzyme-linked immunosorbent assay. Results: AGEs were lower in all BD patients compared to healthy subjects. Depressed patients had approximately two-fold higher S100B levels compared to healthy subjects. Manic and depressed BD patients had increased superoxide dismutase mRNA levels. Apoptosis as measured by BAX/Bcl2 ratio, cytochrome c release, caspase-3 activity was increased in manic and depressed patients compared to healthy subjects. In the depressed patients, S100B levels correlated with cytochrome c release. Conclusions: In conclusion, our study shows decreased AGEs and increased S100B levels and caspase downstream apoptosis in peripheral lymphocytes of BD patients that may underlie disease etiopathogenesis.
Συγγραφείς:
Moutsatsou, P.
Tsoporis, J.N.
Salpeas, V.
Bei, E.
Alevizos, B.
Anagnostara, C.
Izhar, S.
Proteau, G.
Rizos, E.
Hatziagelaki, E.
Toumpoulis, I.K.
Rizos, I.K.
Parker, T.G.
Περιοδικό:
Clinical Chemistry and Laboratory Medicine (CCLM)
Εκδότης:
Walter de Gruyter GmbH
Λέξεις-κλειδιά:
advanced glycation end product; caspase 3; cytochrome c; messenger RNA; protein Bax; protein bcl 2; protein S100B; superoxide dismutase; advanced glycation end product; protein S100B; S100B protein, human, adult; apoptosis; article; bipolar depression; bipolar disorder; bipolar mania; clinical article; controlled study; cross-sectional study; enzyme activity; enzyme linked immunosorbent assay; enzyme release; female; human; human cell; male; mental patient; pathogenesis; pathophysiology; peripheral lymphocyte; priority journal; psychiatric diagnosis; aged; bipolar disorder; blood; lymphocyte; metabolism; middle aged; pathology; young adult, Adult; Aged; Apoptosis; Bipolar Disorder; Female; Glycosylation End Products, Advanced; Humans; Lymphocytes; Male; Middle Aged; S100 Calcium Binding Protein beta Subunit; Young Adult
DOI:
10.1515/cclm-2013-0978
