Περίληψη:
Obesity and cardiovascular disease (CVD) often co-exist, but the pathophysiologic mechanisms that link the two are not fully understood. Lipoprotein-associated phospholipase A2 (Lp-PLA2) is involved in the modification of lipids within atheromatous plaques. Recently, circulating Lp-PLA2 was found to be predictive of thromboembolic episodes in adults, independently of a variety of other potential risk factors, including markers of inflammation, renal function, and hemodynamic stress. However, the function of this lipase and its importance as a biomarker in childhood obesity is much less studied. The aim of the study was to study Lp-PLA2, a non-traditional risk factor of CVD, in obese children. Methods: Sixty-seven lean [39 boys and 28 girls, mean body mass index (BMI) z-score-0.2±0.8] and 66 obese (32 boys and 34 girls, mean BMI z-score 4.4±1.2) age-matched (p=0.251) children, aged 6-12 years, were studied. BMI z-score was calculated based on the Greek BMI growth curves, and children were categorized as obese according to the Cole criteria. All children underwent physical examination and a fasting morning blood sample was obtained for glucose, insulin, lipid profile, and Lp-PLA2 assessment. Plasma concentrations of Lp-PLA2 were determined by a commercially available Lp-PLA2 enzyme-linked immunosorbent assay kit (PLAC Test), while other measurements were performed using standard methods. Results: Plasma Lp-PLA2 levels were significantly higher in obese children (322.5±77.8 ng/mL) compared with normal-weight ones (278.0±64.4 ng/mL, p<0.001). Lp-PLA2 concentrations were significantly correlated with the BMI z-score (p=0.004). Receiver operating characteristic analysis on Lp-PLA2 values resulted in significant areas under the curve (AUC) for distinguishing between obese and normal-weight groups of children (AUC, 0.726; p<0.001). Conclusions: We found significantly higher Lp-PLA2 levels in obese children than lean controls. Interestingly, they all had levels >200 ng/mL, which are considered to correlate with atherosclerosis and a high thromboembolic risk in adults. The positive correlation of Lp-PLA2 with BMI suggests that Lp-PLA2 might be the link between obesity and increased cardiovascular risk, which can be elevated even at a very young age. Measurement of Lp-PLA2 in plasma could therefore represent a further biomarker for assessing increased CVD risk in obese children and adolescents. © 2015 by De Gruyter.
Συγγραφείς:
Sakka, S.
Siahanidou, T.
Voyatzis, C.
Pervanidou, P.
Kaminioti, C.
Lazopoulou, N.
Kanaka-Gantenbein, C.
Chrousos, G.P.
Papassotiriou, I.
Λέξεις-κλειδιά:
1 alkyl 2 acetylglycerophosphocholine esterase; apolipoprotein A1; apolipoprotein B; biological marker; cholesterol; glucose; high density lipoprotein cholesterol; hydrocortisone; insulin; lipid; lipoprotein A; low density lipoprotein cholesterol; triacylglycerol; 1 alkyl 2 acetylglycerophosphocholine esterase; biological marker; lipid, area under the curve; Article; atherosclerosis; blood sampling; body mass; cardiovascular disease; cardiovascular risk; child; childhood obesity; cholesterol blood level; controlled study; correlation analysis; diagnostic test accuracy study; diastolic blood pressure; diet restriction; enzyme linked immunosorbent assay; female; glucose blood level; groups by age; growth curve; human; hydrocortisone blood level; insulin blood level; insulin resistance; lean body weight; lipid blood level; major clinical study; male; physical examination; predictive value; priority journal; receiver operating characteristic; school child; sensitivity and specificity; systolic blood pressure; thromboembolism; triacylglycerol blood level; blood; body mass; enzymology; insulin resistance; obesity, 1-Alkyl-2-acetylglycerophosphocholine Esterase; Biomarkers; Body Mass Index; Child; Female; Humans; Insulin Resistance; Lipids; Male; Obesity
