Τίτλος:
Impact of androgen and dietary advanced glycation end products on female rat liver
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/Aims: Advanced glycation end products (AGEs) have been related to a wide range of liver disorders including hyperandrogenic states such as the Polycystic Ovary Syndrome (PCOS). The aim of the present study is to evaluate the potential impact of dietary glycotoxins exposure and androgen excess on hepatic histology and biochemistry in an androgenized female rat model. Methods: The study population consisted of 80 female Wistar rats, divided in 3 groups, a group of prepubertal (Group A, n=30) and adult rats (Group B, n=20) that were androgenized via subcutaneous implantation of dihydrotestosterone-containing pellets as well as a group of adult non-androgenized rodents (Group C, n=30). All groups were randomly assigned either to a high-AGE or low-AGE diet for 3 months. Results: Rats fed with a high-AGE diet exhibited significantly elevated levels of gamma-glutamyl transferase (γGT) (p≤0.0002) and indices of AGE immunostaining in liver tissue (p<0.01) when compared to the respective low-AGE group, while aspartate aminotransferase (AST) levels were affected only in non-androgenized animals (p=0.0002). Androgenization per se constitutes an aggravating factor as demonstrated by the elevated γGT levels in adult androgenized animals compared to non-androgenized, independent of diet content (p=0.0002) and by the elevated AST and alanine aminotransferase (ALT) levels in low-AGE subgroups (adult androgenized vs. non-androgenized, p=0.0002) followed by increased immunohistochemical AGE deposition in hepatocytes of the latter categories (p=0.0007). Conclusion: The present study suggests that androgens and glycotoxins may contribute synergistically to distort hepatic physiology and function as observed in hyperandrogenic conditions. Open Access License: This is an Open Access article licensed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC) ("http://www.karger.com/OA-license" http://www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. © 2015 The Author(s) Published by S. Karger AG, Basel.
Συγγραφείς:
Palioura, E.
Palimeri, S.
Piperi, C.
Sakellariou, S.
Kandaraki, E.
Sergentanis, T.
Levidou, G.
Agrogiannis, G.
Papalois, A.
Korkolopoulou, P.
Diamanti-Kandarakis, E.
Papavassiliou, A.G.
Περιοδικό:
Cellular Physiology and Biochemistry
Λέξεις-κλειδιά:
advanced glycation end product; alanine aminotransferase; androgen; androstanolone; aspartate aminotransferase; C reactive protein; gamma glutamyltransferase; testosterone; advanced glycation end product; androgen; gamma glutamyltransferase, adult; alanine aminotransferase blood level; animal experiment; animal tissue; Article; aspartate aminotransferase blood level; controlled study; female; gamma glutamyl transferase blood level; hyperandrogenism; liver; liver histology; nonhuman; priority journal; rat; virilization; animal; chemically induced; dietary supplement; disease model; drug effects; enzymology; human; liver; metabolism; ovary polycystic disease; Wistar rat, Androgens; Animals; Dietary Supplements; Disease Models, Animal; Female; gamma-Glutamyltransferase; Glycosylation End Products, Advanced; Humans; Liver; Polycystic Ovary Syndrome; Rats; Rats, Wistar
