Τίτλος:
Mesenchymal derivatives of genetically unstable human embryonic stem cells are maintained unstable but undergo senescence in culture as do bone marrow-derived mesenchymal stem cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Recurrent chromosomal alterations have been repeatedly reported in cultured human embryonic stem cells (hESCs). The effects of these alterations on the capability of pluripotent cells to differentiate and on growth potential of their specific differentiated derivatives remain unclear. Here, we report that the hESC lines HUES-7 and -9 carrying multiple chromosomal alterations produce in vitro mesenchymal stem cells (MSCs) that show progressive growth arrest and enter senescence after 15 and 16 passages, respectively. There was no difference in their proliferative potential when compared with bone marrow-derived MSCs. Array comparative genomic hybridization analysis (aCGH) of hESCs and their mesenchymal derivatives revealed no significant differences in chromosomal alterations, suggesting that genetically altered hESCs are not selected out during differentiation. Our findings indicate that genetically unstable hESCs maintain their capacity to differentiate in vitro into MSCs, which exhibit an in vitro growth pattern of normal MSCs and not that of transformed cells. © Mary Ann Liebert, Inc.
Συγγραφείς:
Karagiannidou, A.
Varela, I.
Giannikou, K.
Tzetis, M.
Spyropoulos, A.
Paterakis, G.
Petrakou, E.
Theodosaki, M.
Goussetis, E.
Kanavakis, E.
Περιοδικό:
Cellular Reprogramming
Λέξεις-κλειδιά:
article; bone marrow derived mesenchymal stem cell; cell aging; cell differentiation; cell growth; cell line; cell proliferation; cell transformation; comparative genomic hybridization; controlled study; embryonic stem cell; genomic instability; hematopoietic stem cell; human; human cell; in vitro study; mesenchymal stem cell; priority journal, Bone Marrow Cells; Cell Aging; Cell Line; Child; Comparative Genomic Hybridization; Embryonic Stem Cells; Female; Genomic Instability; Humans; Male; Mesenchymal Stromal Cells
DOI:
10.1089/cell.2013.0040
