Τίτλος:
14-3-3 gene expression exerts isoform-dependent functions in sinonasal pathophysiology
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The expression profiles of 14-3-3β and θ isoforms, known to exert both oncogenic and antiapoptotic effects, were assessed in different entities of nasal pathophysiology.Flow cytometry and immunohistochemistry were used on paraffin-embedded sections of 51 inverted papillomas (IP), 26 nasal polyps (NP), 9 polyps with IP (NPIP) and 10 specimens of normal epithelium (NE).14-3-3β expression was significantly upregulated in IP as compared with both NP ( p= 0.015) and NE ( p= 0.002). 14-3-3β was also increased in NPIP as compared with NE ( p= 0.008). 14-3-3β cytoplasmic staining was more pronounced in basal cells of the respiratory epithelium although serous glands and the vascular system were often positive as well. High 14-3-3β immunopositivity in IP patients concurred with increased proliferative activity shown by PCNA immunostaining ( p= 0.04). Expression of 14-3-3θ was also found increased in IP and NPIP patients, compared to NP ( p= 0.005, p= 0.002 respectively) and NE ( p= 0.004 and p= 0.001 respectively). 14-3-3θ cytoplasmic immunopositivity was detected in columnar epithelium, particularly in basal and subluminal cells, whereas no immunoreactivity was observed in NP and NE.Our results demonstrate differential expression of 14-3-3β and θ isoforms in sinonasal pathophysiology, supporting their implication, respectively, in the proliferative and inflammatory process engaged in the formation of IP. © 2013 Elsevier GmbH.
Συγγραφείς:
Kataki, A.
Alevizos, L.
Lazaris, A.
Glustianou, G.
Derventzi, A.
Giotakis, E.
Konstadoulakis, M.
Yiotakis, I.
Περιοδικό:
PATHOLOGY RESEARCH AND PRACTICE
Λέξεις-κλειδιά:
cycline; protein 14 3 3; stratifin; isoprotein; protein 14 3 3; YWHAB protein, human, 14 3 3 beta gene; 14 3 3 gene; 14 3 3 sigma gene; adult; aged; apoptosis; article; basal cell; cell proliferation; controlled study; cytoplasm; female; flow cytometry; gene; gene expression; human; human tissue; immunohistochemistry; inverted papilloma; major clinical study; male; nose polyp; paranasal sinus disease; respiratory epithelium; upregulation; adolescent; cohort analysis; epithelium cell; gene expression regulation; metabolism; middle aged; nose mucosa; nose tumor; papilloma; pathophysiology; very elderly; young adult, 14-3-3 Proteins; Adolescent; Adult; Aged; Aged, 80 and over; Apoptosis; Cell Proliferation; Cohort Studies; Epithelial Cells; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Middle Aged; Nasal Mucosa; Nasal Polyps; Nose Neoplasms; Papilloma; Papilloma, Inverted; Protein Isoforms; Young Adult
DOI:
10.1016/j.prp.2013.10.006
