Τίτλος:
Modeling, substrate docking, and mutational analysis identify residues essential for the function and specificity of a eukaryotic purine-cytosine NCS1 transporter
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: The purine-cytosine FcyB transporter is a prototype member of the NCS1 family. Results: Using homology modeling, substrate docking, and rational mutational analysis, we identify residues critical for function and specificity. Conclusion: Important aspects concerning the molecular mechanism and evolution of transporter specificity are revealed. Significance: The first systematic approach on structure-function-specificity relationships in a eukaryotic NCS1 member is shown. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.
Συγγραφείς:
Krypotou, E.
Kosti, V.
Amillis, S.
Myrianthopoulos, V.
Mikros, E.
Diallinas, G.
Περιοδικό:
Journal of Biological Chemistry
Λέξεις-κλειδιά:
Homology modeling; Molecular mechanism; Mutational analysis, Docking, Rational functions, asparagine; cytosine; fungal protein; NCS1 transporter; nucleobase transporter; proline; protein FcyB; serine; tryptophan; unclassified drug, article; Aspergillus nidulans; binding affinity; controlled study; enzyme activity; enzyme specificity; enzyme substrate; molecular docking; molecular dynamics; molecular evolution; mutational analysis; nonhuman; priority journal; protein binding; protein expression; protein function; protein protein interaction; protein structure; structure activity relation; structure analysis, Adenine; Amino Acid Motifs; Amino Acid Substitution; Aspergillus nidulans; Conserved Sequence; Cytosine; Fungal Proteins; Guanine; Hydrogen Bonding; Hypoxanthine; Kinetics; Molecular Docking Simulation; Molecular Dynamics Simulation; Mutagenesis, Site-Directed; Phenotype; Protein Binding; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Structural Homology, Protein; Substrate Specificity; Symporters, Eukaryota
DOI:
10.1074/jbc.M112.400382
