Τίτλος:
Diagnostic and prognostic significance of human kallikrein 11 (KLK11) mRNA expression levels in patients with laryngeal cancer
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objectives: Human kallikrein 11 gene (KLK11) encodes a secreted serine protease. In view of its diagnostic and prognostic strength in many malignancies, we investigated the mRNA expression levels of KLK11 in laryngeal tissues in order to unveil its clinical usefulness in laryngeal cancer. Design and methods: KLK11 expression was quantified in 163 tissue samples from 105 laryngeal cancer patients with the development of a highly sensitive real-time PCR methodology, using SYBR Green® chemistry. Results: KLK11 expression in laryngeal cancer specimens of primary or recurrent nature was significantly inferior compared with their non-malignant counterparts (P< 0.001 and P= 0.026, respectively), a finding of immense diagnostic value as illustrated in the ROC curve analyses (P< 0.001). Survival analysis showed that patients harboring KLK11-positive tumors had a significantly decreased risk of death (HR = 0.26, P= 0.042). Conclusions: Our data recommend KLK11 mRNA expression as a novel and independent biomarker in laryngeal cancer for diagnostic and prognostic purposes. © 2012 The Canadian Society of Clinical Chemists.
Συγγραφείς:
Patsis, C.
Yiotakis, I.
Scorilas, A.
Περιοδικό:
Clinical Biochemistry
Λέξεις-κλειδιά:
kallikrein; kallikrein 11; messenger RNA; unclassified drug, adult; aged; article; cancer diagnosis; cancer prognosis; cancer risk; cancer survival; controlled study; diagnostic value; disease free survival; female; gene expression; green chemistry; human; human cell; human tissue; larynx cancer; major clinical study; male; overall survival; priority journal; real time polymerase chain reaction; receiver operating characteristic, Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell; Female; Fluorescent Dyes; Gene Expression Regulation, Neoplastic; Humans; Laryngeal Neoplasms; Male; Middle Aged; Organic Chemicals; Prognosis; Real-Time Polymerase Chain Reaction; Retrospective Studies; RNA, Messenger; ROC Curve; Serine Endopeptidases; Survival Analysis; Tumor Markers, Biological
DOI:
10.1016/j.clinbiochem.2012.03.005
