Τίτλος:
Expression of antiapoptosis gene survivin in luteinized ovarian granulosa cells of women undergoing IVF or ICSI and embryo transfer: clinical correlations
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: The purpose of the study was to determine the incidence of survivin gene expression in human granulosa cells during ovarian stimulation in Greek women with normal FSH levels, undergoing IVF or ICSI and to discover any correlation between levels of gene expression and clinical parameters, efficacy of ovulation or outcomes of assisted reproduction.Methods: Twenty nine women underwent ovulation induction for IVF or ICSI and ET with standard GnRH analogue-recombinant FSH protocol. Infertility causes were male and tubal factor. Cumulus-mature oocyte complexes were denuded and the granulosa cells were analyzed for each patient separately using quantitative reverse transcription polymerase chain reaction analysis for survivin gene expression with internal standard the ABL gene.Results: The ABL and survivin mRNA were detected in granulosa cells in 93.1%. The expression levels of survivin were significantly lower in normal women (male infertility factor) compared to women with tubal infertility factor (p = 0.007). There was no additional statistically significant correlation between levels of survivin expression and estradiol levels or dosage of FSH for ovulation induction or number of dominant follicles aspirated or number of retrieved oocytes or embryo grade or clinical pregnancy rates respectively.Conclusions: High levels of survivin mRNA expression in luteinized granulosa cells in cases with tubal infertility seem to protect ovaries from follicular apoptosis. A subpopulation of patients with low levels of survivin mRNA in granulosa cells might benefit with ICSI treatment to bypass possible natural barriers of sperm-oocyte interactions. © 2012 Varras et al.; licensee BioMed Central Ltd.
Συγγραφείς:
Varras, M.
Polonifi, K.
Mantzourani, M.
Stefanidis, K.
Papadopoulos, Z.
Akrivis, C.
Antsaklis, A.
Περιοδικό:
Reproductive Biology and Endocrinology
Λέξεις-κλειδιά:
buserelin; chorionic gonadotropin; complementary DNA; estradiol; follitropin; gonadorelin; luteinizing hormone; messenger RNA; Muellerian inhibiting factor; progesterone; prolactin; recombinant follitropin; survivin, adult; apoptosis; article; clinical article; embryo transfer; female; fertilization in vitro; gene expression; granulosa cell; hormone determination; human; human cell; infertility; infertility therapy; intracytoplasmic sperm injection; ovary cell; ovulation induction; pregnancy rate; quantitative analysis; radioimmunoassay; reverse transcription polymerase chain reaction; treatment outcome, Adult; Embryo Transfer; Female; Fertilization in Vitro; Granulosa Cells; Humans; Infertility, Female; Inhibitor of Apoptosis Proteins; Male; Ovulation Induction; Pregnancy; RNA, Messenger; Sperm Injections, Intracytoplasmic
DOI:
10.1186/1477-7827-10-74
