Τίτλος:
Extracellular matrix-associated (GAGs, CTGF), angiogenic (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in type 1 diabetes mellitus nephropathy
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: The aim of the present study was to evaluate the role of extracellular matrix-associated glycosaminoglycans (GAGs), connective tissue growth factor (CTGF), angiogenic vascular endothelial growth factor (VEGF) and inflammatory factors (MCP-1, CD40, IFN-γ) in the development of diabetic nephropathy in type 1 diabetes (T1DM). Methods: Plasma and urine samples from 30 T1DM patients and 20 healthy controls were used to measure the levels of CTGF, VEGF, MCP-1, CD40 and IFN-γ by ELISA. Plasma and urine GAGs were measured using a spectrophotometric method. Results: Plasma levels of GAGs, CD40 and MCP-1 and urine levels of GAGs and CTGF were significantly elevated in normoalbuminuric T1DM patients. A tendency to higher plasma VEGF levels was found in patients compared to controls. The urine/plasma GAGs ratio of T1DM patients was almost similar to that of healthy subjects (HS), whereas the urine/plasma CTGF ratio was about three times greater in diabetic patients compared to HS. Conclusions: Conclusively, increased GAGs and CTGF excretion are evident in T1DM normoalbuminuric juveniles, possibly reflecting early renal injury signs, before the initiation of albuminuria. © 2011 by Walter de Gruyter.
Συγγραφείς:
Ellina, O.
Chatzigeorgiou, A.
Kouyanou, S.
Lymberi, M.
Mylona-Karagianni, C.
Tsouvalas, E.
Kamper, E.F.
Περιοδικό:
Clinical Chemistry and Laboratory Medicine (CCLM)
Λέξεις-κλειδιά:
CD40 antigen; connective tissue growth factor; gamma interferon; glycosaminoglycan; monocyte chemotactic protein 1; vasculotropin, adolescent; albuminuria; angiogenesis; article; child; clinical article; controlled study; correlation analysis; diabetic nephropathy; disease course; disease duration; enzyme linked immunosorbent assay; extracellular matrix; female; human; inflammation; insulin dependent diabetes mellitus; kidney injury; male; prediction; priority journal; protein blood level; protein urine level; school child; spectrophotometry, Antigens, CD40; Biological Markers; Chemokine CCL2; Child; Diabetes Mellitus, Type 1; Diabetic Nephropathies; Extracellular Matrix; Female; Glycosaminoglycans; Humans; Interferon-gamma; Male; Vascular Endothelial Growth Factor A
DOI:
10.1515/CCLM.2011.881
