Τίτλος:
Hypoxemic resuscitation from hemorrhagic shock prevents lung injury and attenuates oxidative response and IL-8 overexpression
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
We investigated whether hypoxemic resuscitation from hemorrhagic shock prevents lung injury and explored the mechanisms involved. We subjected rabbits to hemorrhagic shock for 60 min by exsanguination to a mean arterial pressure of 40 mm Hg. By modifying the fraction of the inspired oxygen, we performed resuscitation under normoxemia (group NormoxRes, PaO2 = 95-105 mm Hg) or hypoxemia (group HypoxRes, PaO2 = 35-40 mm Hg). Animals not subjected to shock constituted the sham group (P aO2 = 95-105 mm Hg). We performed bronchoalveolar lavage (BAL) fluid, lung wet-to-dry weight ratio, and morphological studies. U937 monocyte-like cells were incubated with BAL fluid from each group. Cell peroxides, malondialdehyde, proteins, and cytokines in the BAL fluid were lower in sham than in shocked animals and in HypoxRes than in NormoxRes animals. The inverse was true for ascorbic acid and reduced glutathione. Lung edema, lung neutrophil infiltration, myeloperoxidase, and interleukin (IL)-8 gene expression were reduced in lungs of HypoxRes compared with NormoxRes animals. A colocalized higher expression of IL-8 and nitrotyrosine was found in lungs of NormoxRes animals compared to HypoxRes animals. The BAL fluid of NormoxRes animals compared with HypoxRes animals exerted a greater stimulation of U937 monocyte-like cells for proinflammatory cytokine release, particularly for IL-8. In the presence of p38-MAPK and Syk inhibitors and monosodium urate crystals, IL-8 release was reduced. We conclude that hypoxemic resuscitation from hemorrhagic shock ameliorates lung injury and reduces oxygen radical generation and lung IL-8 expression. © 2010 Elsevier Inc. All rights reserved.
Συγγραφείς:
Douzinas, E.E.
Betrosian, A.
Giamarellos-Bourboulis, E.J.
Tasoulis, M.-K.
Prigouris, P.
Livaditi, O.
Andrianakis, I.
Goutas, N.
Vlachodimitropoulos, D.
Pelekanou, A.
Villiotou, V.
Legakis, I.
Chrousos, G.P.
Περιοδικό:
FREE RADICAL BIOLOGY AND MEDICINE
Λέξεις-κλειδιά:
3 nitrotyrosine; ascorbic acid; cytokine; glutathione; interleukin 8; malonaldehyde; peroxide; protein; reactive oxygen metabolite; synaptophysin; urate, animal cell; animal experiment; animal model; article; cell strain U937; controlled study; cytokine release; enzyme activity; hemorrhagic shock; hypoxemia; lung edema; lung injury; lung lavage; lung parenchyma; male; neutrophil chemotaxis; nonhuman; oxidative stress; priority journal; protein expression; rabbit; resuscitation, Animals; Anoxia; Bronchoalveolar Lavage Fluid; Cytokines; Disease Models, Animal; Humans; Immunoenzyme Techniques; Interleukin-8; Lung Injury; Male; Neutrophils; Peroxidase; Rabbits; Reactive Oxygen Species; Resuscitation; Shock, Hemorrhagic; U937 Cells, Animalia; Oryctolagus cuniculus
DOI:
10.1016/j.freeradbiomed.2010.10.712
