Τίτλος:
Transcripts of PTTG and growth factors bFGF and IGF-1 are correlated in pituitary adenomas
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The reasons for the increase of pituitary tumor-transforming gene (PTTG) transcripts in about 90% of pituitary adenomas are still not fully understood, although upregulation by basic fibroblast growth factor (bFGF) has been discussed as a potential cause. A possible influence of the Insulin like Growth Factor 1 (IGF-1) might be of interest, since this protein is also synthesized in most pituitary adenomas. Moreover, the principal regulation of the PTTG gene by IGF-1 and Insulin has been demonstrated in astrocytoma and breast cancer cells. We analyzed a large group (103 patients) of unselected clinical pituitary adenoma samples. From total RNA of frozen tumor samples (all subtypes) cDNA (complementary DNA) was synthesized and transcripts of PTTG, bFGF, IGF-1 were measured by Real-Time-PCR. Not only mRNA (messenger RNA) levels of bFGF, but also of IGF-1, correlated strongly with PTTG transcripts. This result was obtained, when all pituitary adenoma samples were included in the statistical calculations, irrespective of their subclassification. Our study suggests a connection between PTTG and IGF-1 in pituitary adenomas. © J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart New York.
Συγγραφείς:
Chamaon, K.
Kanakis, D.
Mawrin, C.
Dietzmann, K.
Kirches, E.
Περιοδικό:
Experimental and Clinical Endocrinology and Diabetes
Λέξεις-κλειδιά:
basic fibroblast growth factor; complementary DNA; messenger RNA; RNA; securin; somatomedin C, article; astrocytoma; breast cancer; calculation; cancer cell; controlled study; frozen section; gene control; genetic transcription; human; human cell; hypophysis adenoma; immunohistochemistry; major clinical study; priority journal; reverse transcription polymerase chain reaction, Adenoma; Fibroblast Growth Factor 2; Gene Expression Regulation, Neoplastic; Humans; Insulin-Like Growth Factor I; Neoplasm Proteins; Pituitary Gland; Pituitary Neoplasms; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger
DOI:
10.1055/s-0029-1215588
