Τίτλος:
Genome-wide transcriptome profile of the human osteosarcoma Sa OS and U-2 OS cell lines
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
With the use of genome-wide cDNA microarrays, we investigated the transcriptome profile of the human osteosarcoma Sa OS and U-2 OS cell lines. In all, 1,098 chip entries were differentially regulated in the two cell lines; of these, 796 entries corresponded to characterized mRNAs. The identified genes are mostly expressed in epithelial tissues and localize on chromosomes 1, 10, and 20. Furthermore, signaling cascades for cell cycle, glycolysis, and gluconeogenesis, the p53 pathway, cell communication, and focal adhesion were found to be differently regulated in the two cell lines. The transcriptome profiles reported here provide novel information about the considerable molecular differences between these two widely used human osteosarcoma cell lines. © 2010 Elsevier Inc. All rights reserved.
Συγγραφείς:
Trougakos, I.P.
Chondrogianni, N.
Amarantos, I.
Blake, J.
Schwager, C.
Wirkner, U.
Ansorge, W.
Gonos, E.S.
Περιοδικό:
CANCER GENETICS AND CYTOGENETICS
Λέξεις-κλειδιά:
messenger RNA; protein p53; transcriptome, article; cancer cell culture; cell communication; cell cycle; chromosome 1; chromosome 10; chromosome 20; controlled study; epithelium; focal adhesion; gene control; gene expression; gene identification; gene location; genetic analysis; genetic difference; genetic variability; gluconeogenesis; glycolysis; human; human cell; oncogene; osteosarcoma; priority journal; signal transduction, Base Sequence; Bone Neoplasms; Cell Line, Tumor; DNA Primers; DNA, Complementary; Gene Expression Profiling; Humans; Oligonucleotide Array Sequence Analysis; Osteosarcoma; Polymerase Chain Reaction; RNA, Messenger
DOI:
10.1016/j.cancergencyto.2009.09.012
