Τίτλος:
A modified chromatin-immunoprecipitation protocol for silkmoth ovarian follicular cells reveals C/EBP and GATA binding modes on an early chorion gene promoter
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Standard Chromatin immunoprecipitation protocols have been designed to suit studies performed on cell line cultures or yeast cells growing in liquid cultures. In these cases cross-linking/fixation takes place directly in the growing medium of the cells by the addition of a general fixation reagent. When applied on whole isolated silkmoth follicles, this procedure results in poor release of follicular cells from the basal membrane and lower yield of cross-linked chromatin. We present a modification to the standard protocol, where detachment of follicular cells from the basal membrane of the egg and nuclei isolation precedes formaldehyde-mediated cross-linking. We also discuss application of the modified method for the identification of distinct BmC/EBP and BmGATAβ binding modes on a chorion gene promoter from the Er1.A/B early gene pair. © 2008 Springer Science+Business Media B.V.
Συγγραφείς:
Papantonis, A.
Lecanidou, R.
Περιοδικό:
Current Molecular Biology Reports
Λέξεις-κλειδιά:
Animals; Bombyx; CCAAT-Enhancer-Binding Proteins; Chorion; Chromatin Immunoprecipitation; Female; GATA Transcription Factors; Ovarian Follicle; Promoter Regions, Genetic; Time Factors, Bombyx
DOI:
10.1007/s11033-008-9236-1
