Τίτλος:
HOX-1 and COX-2: Two differentially regulated key mediators of skeletal myoblast tolerance under oxidative stress
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The exact physiological role of oxidative stress as a primary cause for skeletal muscle pathological conditions involving muscle degeneration remains elusive. Therefore, the present study was performed so as to decipher the signalling pathways orchestrating the potential cytoprotective role of heme oxygenase 1 (HOX-1) as well as cyclooxygenase 2 (COX-2) in skeletal myoblasts exposed to H2O2. Cell treatment with H2O 2 (0.5 mM) resulted in a time-and dose-dependent response of HOX-1 and COX-2 mRNA and protein levels, with ERK1/2, p38-MAPK and MSK1 found to mediate these effects. Furthermore, Src and JNKs blockade attenuated COX-2 response. Collectively, these novel findings highlight for the first time HOX-1 and COX-2 fundamental contribution to skeletal myoblast tolerance under oxidative stress, since their inhibition significantly attenuated viability of skeletal myoblasts. The data also delineate the various effectors regulating HOX-1 and COX-2 expression, probably alleviating muscle degeneration in related disorders. © 2010 Informa UK Ltd.
Συγγραφείς:
Aggeli, I.-K.
Kefaloyianni, E.
Beis, I.
Gaitanaki, C.
Περιοδικό:
Free Radical Research
Λέξεις-κλειδιά:
cyclooxygenase 2; heme oxygenase 1; hydrogen peroxide; messenger RNA; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; mitogen and stress activated kinase 1; phosphotransferase; protein tyrosine kinase; stress activated protein kinase; unclassified drug, animal cell; article; cell protection; cell stress; cell viability; controlled study; enzyme inhibition; enzyme regulation; mouse; muscle atrophy; myoblast; nonhuman; nucleotide sequence; oxidative stress; protein expression; signal transduction; skeletal muscle, Animals; Blotting, Western; Cells, Cultured; Cyclooxygenase 2; Electrophoretic Mobility Shift Assay; Fluorescent Antibody Technique; Gene Expression; Gene Expression Regulation; Heme Oxygenase-1; Hydrogen Peroxide; Mice; Myoblasts, Skeletal; Oxidants; Oxidative Stress; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Signal Transduction
DOI:
10.3109/10715761003742985
