Τίτλος:
Effect of hepatic stimulator substance (HSS) on cadmium-induced acute
hepatotoxicity in the rat liver
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The hepatoprotective effect of HSS against cadmium-induced liver injury
was investigated. Rats were intoxicated with a dose of cadmium (3.5
mg/kg b.w.). The rats were treated with normal saline (group I) or HSS
(100 mg protein/kg b.w.; group II) 2 hr later and killed at different
time points. Hematoxylin-eosin (HE) sections were assessed for necrosis,
apoptosis, peliosis, mitoses, and inflammatory infiltration. Serum
enzyme activities were assayed. Apoptosis was quantified by the Tunel
technique. Thymidine kinase activity and the rate of [H-3] thymidine
incorporation into DNA were also assayed. Necrosis, hepatocyte
apoptosis, and peliosis were minimized in HSS-treated rats (group II).
Nonparenchymal cell apoptosis and liver regeneration were not
quantitively altered in the HSS-treated group, though the time profile
was different. HSS protects hepatocytes against cadmium-induced
necrosis, apoptosis, and peliosis. Apoptosis was the major type of cell
death for nonparenchymal liver cells and strongly correlated with the
extent of peliosis. Interactions between hepatocytes and nonparenchymal
liver cells seem to be important for the genesis of hepatic trauma in
acute cadmium hepatotoxicity.
Συγγραφείς:
Tzirogiannis, KN
Panoutsopoulos, GI
Demonakou, MD
Hereti, RI
and Alexandropoulou, KN
Mykoniatis, MG
Περιοδικό:
Digestive Diseases and Sciences
Λέξεις-κλειδιά:
hepatic stimulator substance (HSS); cadmium toxicity; liver injury;
apoptosis; necrosis; peliosis; hepatocytes; nonparenchymal cells
DOI:
10.1023/B:DDAS.0000034566.43582.53
