Τίτλος:
Mononucleotide markers of microsatellite instability in carcinomas of
the urinary bladder
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Aims. To determine the presence of microsatellite instability (MSI) and
to assess the expression of the human mismatch repair (MMR) gene
products hMLH1 and hMSH2 in primary transitional cell carcinomas (TCCs)
of the urinary bladder in relation to clinico-pathological parameters.
Methods. Seventy-two cases of primary TCC were screened for the presence
of alterations in MSI markers by molecular techniques and evaluated
immunohistochemically for the expression of hMLH1 and hMSH2 proteins.
Clinical data were available in 70 cases. The percentage of MSI rose to
16.6%.
Results. Reduced (<20%) hMLH1 expression was closely related to the
presence of MSI (p = 0.0004). Neither MMR proteins nor MSI was
associated with grade, stage, papillary status. Clinical outcome
analysed as a function of MSI did not show significant differences in
terms of both disease-free and overall survival. Reduced hMLH1
expression was a significant predictor of shorter disease-free survival
in univariate and multivariate analysis.
Conclusions. The presence of MSI is not related to classical
clinico-pathological parameters in TCCs, nor does it appear to be of
prognostic significance. hMLH1 was an important indicator for
recurrence. (C) 2004 Elsevier Ltd. All rights reserved.
Συγγραφείς:
Saetta, AA
Goudopoulou, A
Korkolopoulou, P
Voutsinas, G and
Thomas-Tsagli, E
Michalopoulos, NV
Patsouris, E
Εκδότης:
Elsevier Sci Ltd, Exeter, United Kingdom
Λέξεις-κλειδιά:
MSI; PCR; bladder cancer; BAT-26; TGF-beta RII; MMR
DOI:
10.1016/j.ejso.2004.04.015
