Τίτλος:
Characterisation of the NUCKS gene on human chromosome 1q32.1 and the
presence of a homologous gene in different species
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The NUCKS gene is located on human chromosome 1q32.1 and consists of
seven exons and six introns. The gene lacks a TATA box but contains two
Inr elements, two GC boxes, and one consensus-binding site for E2F-1.
NUCKS is expressed in all human adult and foetal tissues investigated,
and has all the features of being a housekeeping gene. Both data
searches and Western immunoblotting experiments show that a homologous
protein is present in fish, amphibians, and birds but not in insects and
yeast, suggesting that NUCKS is a vertebrate specific gene. In all the
species investigated, the protein contains several consensus
phosphorylation sites for cyclin-dependent kinases and CK-2, and we have
shown that the fish protein (like mammalian NUCKS) indeed is a substrate
for CDK1 and CK-2 in vitro. The NUCKS protein is also conserved with
respect to a DNA-binding domain previously characterised in mammals, and
two putative bipartite nuclear localisation signals. (C) 2004 Elsevier
Inc. All rights reserved.
Συγγραφείς:
Grundt, K
Haga, IV
Aleporou-Marinou, V
Drosos, Y
Wanvik,
B
Ostvold, AC
Περιοδικό:
Biochemical and Biophysical Research Communications
Εκδότης:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Λέξεις-κλειδιά:
NUCKS; chromosome 1; vertebrates; Inr elements
DOI:
10.1016/j.bbrc.2004.08.153
