Τίτλος:
Expression of the proliferation-associated nuclear protein lNUB-1 and
its relationship with microvascular density in bone marrow biopsies of
patients with myelodysplastic syndromes
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The myelodysplastic syndromes (MDS) are a group of disorders
characterized by dysplastic hemopoiesis and an increased risk of
leukemic transformation. The process of angiogenesis has been implicated
in the pathogenesis and evolution of MDS. In this study the
proliferative activity and extent of angiogenesis was examined in bone
marrow samples from 54 patients with MDS in relation to
clinicopathologic, features. Cellular proliferation and microvascular
density (MVD) were examined immunohistochemically, using the monoclonal
antibody MIB-I (Ki-67) and an anti-CD34 monoclonal antibody
respectively. Serum concentrations of interleukin-6 (IL-6) were measured
by ELISA. The results showed that the MIB-1 Labeling Index (MIB-1 LI),
MVD and IL-6 increased significantly with advancing severity of disease.
Among the MDS-FAB subtypes. MIB-1 LI, MVD and IL-6 were significantly
higher in RAEB-t, RAEB and CMML in comparison to RA and RARS (p < 0.0001
in all cases). Similarly, MIB-1 and MVD were increased in patients with
score 3 in comparison to scores 0 and 1 in the IPSS system (p < 0.05).
All parameters studied were significantly higher in patients versus
controls. We conclude that cellular proliferative activity and
angiogenesis are associated with disease progression in MDS patients.
Συγγραφείς:
Alexandrakis, MG
Passam, FH
Kyriakou, DS
Dambaki, C and
Katrinakis, GE
Tsirakis, G
Konsolas, J
Stathopoulos, EN
Περιοδικό:
Journal of Molecular Histology
Λέξεις-κλειδιά:
angiogenesis; MIB-1 labeling index; microvascular density;
proliferation; Ki-67; MDS
DOI:
10.1007/s10735-004-2341-0
