Τίτλος:
Cyclin D1 protein tissue detection in laryngeal cancer
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Cyclin D1 (CCND1) is a set of periodic regulatory proteins that is
believed to govern cell cycle transit from G1 into S phase.
Overexpression of CCND1 leads to abnormal cellular proliferation which
underlies processes of tumorigenesis; CCND1 can thus function as a
cooperative oncogene in cell transformation. In the present study we
investigate the immunohistochemical expression of CCND1 in a
well-documented series of 58 laryngeal squamous cell carcinomas (LSCC)
and search for statistical associations between CCND1 index and various
clinicopathological parameters including several immunomarkers’
expression as well as patients’ disease-free survival. Tissue sections
from archival paraffin blocks were stained using the
avidin-biotin-peroxidase complex method; the H-295 rabbit polyclonal
antibody was applied at dilution of 1: 150. The percentage of CCND1
immunoreactive tumor cells for each tumor was counted by an image
analysis system. CCND1 staining was confined to cell nuclei and, in the
examined samples, ranged from undetectable (i.e. 0% of tumor cells, n =
6) to the majority of tumor cells (i.e. 89% of tumor cells) with mean
value: 15.73%. In tumor adjacent, non invasive lesions, strong CCND1
staining was noticed in areas with cellular atypia. In cases with nodal
metastases, no change in CCND1 expression in the nodal metastases
compared with the primary tumors was observed. p53 protein accumulation
in malignant cells was positively linked with CCND1 index (Mann-Whitney
U: 205.5, p = 0.034). CCND1 expression appears to be an early event in
processes of tumorigenesis and tumor progression in some LSCC. Apart
from p53 protein accumulation, CCND1 immunohistochemical expression does
not seem to correlate with nodal metastasis, disease recurrence or any
other clinicopathological prognostic indicator. Copyright (C) 2005 S.
Karger AG, Basel.
Συγγραφείς:
Segas, JV
Lazaris, AC
Nikolopoulos, TP
Kavantzas, NG and
Lendari, IE
Tzagkaroulakis, AM
Patsouris, ES
Ferekidis, EA
Περιοδικό:
ORL: Journal for Oto - Rhino - Laryngology and Its Related Specialties
Λέξεις-κλειδιά:
cyclin D1; immunohistochemistry; p53 protein; laryngeal cancer
