Τίτλος:
Tuberculous effusion: ADA activity correlates with CD4+cell numbers in
the fluid and the pleura
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Adenosine deaminase (ADA) is a commonly used marker in the
diagnosis of tuberculous effusion and there is evidence that its
production is linked to T cells and monocytes. Data on the correlation
between ADA and T cells or macrophages in tuberculous effusions are
conflicting. Furthermore, no studies have examined a possible
correlation between pleural tissue infiltration and ADA. Objectives: We
undertook this study to examine cell subsets in the fluid and the pleura
in tuberculous effusion and their correlation to ADA. The use of cell
subsets as a marker in the differential diagnosis was also examined.
Methods: Pleural fluid from 36 patients with tuberculous and 34 patients
with malignant effusion as well as pleural tissue biopsies from 16
patients with tuberculous pleurisy were examined. The APAAP and the
avidin-biotin complex immunocytochemical methods were used to examine
CD4+ T cells and macrophages (CD68+), while ADA activity was measured by
the Giusti colorimetric method. Results: Our results showed that, in
pleural fluid, CD4+ cells and ADA were significantly higher in
tuberculous compared to malignant effusion (p < 0.001 for all
measurements). In pleural tissue biopsies, macrophages were the
predominant cells but CD4+ T cells were also abundant. A significant
correlation was found between ADA and CD4+ numbers in pleural fluid and
tissue ( r = 0.45, p < 0.01; r = 0.75, p < 0.001, respectively). ADA had
high sensitivity and specificity for differential diagnosis while cell
subsets did not. Conclusions: These results indicate that ADA activity
correlates to CD4+ T cell infiltration in the pleura and the fluid.
Moreover, ADA but no cell subsets may be used as markers of tuberculous
effusion. Copyright (C) 2005 S. Karger AG, Basel.
Συγγραφείς:
Gaga, M
Papamichalis, G
Bakakos, P
Latsi, P
Samara, I
and Koulouris, NG
Alchanatis, N
Orphanidou, D
Περιοδικό:
RESPIRATION PHYSIOLOGY
Λέξεις-κλειδιά:
T cells; adenosine deaminase; macrophages; tuberculosis; pleural
effusion
