Περίληψη:
Male Wistar rats were randomized to receive ethanol (2.5 ml/kg by
gastric intubation every 8 hr; group I), equal volumes of isocaloric to
ethanol sucrose solution (group II), or ethanol and HSS (100 mg/kg
intraperitoneally 10 and 16 hr after partial hepatectomy; groups III and
IV, respectively) for up to 96 hr after partial hepatectomy, with
ethanol administration starting 1 hr prior to partial hepatectomy.
Animals were killed at 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 60,
and 96 hr after partial hepatectomy. The rate of liver regeneration was
evaluated by the mitotic index in H&E-stained sections, immunochemical
detection of Ki67 nuclear antigen, rate of [H-3]thymidine
incorporation into hepatic DNA, and liver thymidine kinase enzymatic
activity. The biological activity of HSS in groups I and II rats was
evaluated using a bioassay. Ethanol administration arrested liver
regeneration during the first 32 hr after partial hepatectomy and
suppressed HSS activity throughout the period examined. Liver
regeneration progressed after 32 hr despite the low levels of HSS
activity. HSS administration at 10 and 16 hr reversed liver regeneration
arrest induced by ethanol. Acute ethanol administration induces cell
cycle arrest during the first 32 hr after partial hepatectomy and
suppression of HSS biological activity seems to contribute to this
effect. HSS administration reversed the inhibitory effect of ethanol on
liver regeneration and caused synchronized entrance of hepatocytes in
the S phase of the cell cycle. HSS seems to participate in the network
of growth factors controlling the G1/S cell cycle checkpoint.
Συγγραφείς:
Kondili, VG
Tzirogiannis, KN
Androutsos, CD
Papadimas, GK
and Demonakou, MD
Hereti, RI
Manta, GA
Kourentzi, KT and
Triantaphyllou, MI
Panoutsopoulos, GI
